MREF2 - Plant Editosome Database - BIG Data Center

MREF2 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	MREF2
Synonym:	NA
Description:	pentatricopeptide (PPR) repeat protein;(source:Araport11)
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	At2g41080 (Arabidopsis thaliana)
Edited Gene(s):	atp1
Editing Type(s):	C-to-U (3)
Publication(s):	[1] Identification of new pentatricopeptide repeat proteins, MREF1 and 2, involved in mitochondrial RNA editing, using computational target RNA prediction, Plant Biotechnol (Tokyo), 2020. [PMID=32362757]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

At2g41080

Mitochondrion

1292

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	WT	No treatment	No treatment	No treatment	NA	Seedling	2 weeks	Sanger sequencing	100.00%	Complete	None	32362757
SALK_087125C	SALK_087125C	T-DNA insertion	The T-DNA insertion was located at the vicinity of the stop codon in coding regions	Homozygous	Two-week-old seedlings of the gene-deficient lines displayed no visible aberrant phenotypes under these experimental conditions.	Seedling	2 weeks	Sanger sequencing	42.11%	Medium	Decreased	32362757
SALK_087125C	Complemented	Complementation	PPR gene-deficient lines were transformed using the floral dip method, with a construct that expresses the corresponding wild-type PPR protein under the control of the A. thaliana ubiquitin-10 promote	Homozygous	NA	Seedling	2 weeks	Sanger sequencing	100.00%	Complete	Restored	32362757

Last update: Jul 2021 (version 1.0)