MREF2 - Plant Editosome Database - BIG Data Center

MREF2 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	MREF2
Synonym:	Mitochondrial RNA-editing factor 2
Description:	Pentatricopeptide (PPR) repeat protein;(source:Araport11)
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT2G41080 (Arabidopsis thaliana)
Edited Gene(s):	atp1
Editing Type(s):	C-to-U (3)
Publication(s):	[1] Identification of new pentatricopeptide repeat proteins, MREF1 and 2, involved in mitochondrial RNA editing, using computational target RNA prediction., Plant biotechnology (Tokyo, Japan), 2020. [PMID=32362757]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G41080

Mitochondrion

1292

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	WT	Control	Control	Control	NA	Seedling	2 weeks	Sanger sequencing	100.00%	Complete	None	32362757
SALK_087125C	SALK_087125C	T-DNA insertion	The T-DNA insertion was located at the vicinity of the stop codon in coding regions	Homozygous	Two-week-old seedlings of the gene-deficient lines displayed no visible aberrant phenotypes under these experimental conditions.	Seedling	2 weeks	Sanger sequencing	42.11%	Medium	Decreased	32362757
SALK_087125C	Complemented	Complementation	PPR gene-deficient lines were transformed using the floral dip method, with a construct that expresses the corresponding wild-type PPR protein under the control of the A. thaliana ubiquitin-10 promote	Homozygous	NA	Seedling	2 weeks	Sanger sequencing	100.00%	Complete	Restored	32362757

Last update: Jul 2021 (version 1.0)