MREF2 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: MREF2
Synonym: Mitochondrial RNA-editing factor 2
Description: Pentatricopeptide (PPR) repeat protein;(source:Araport11)
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT2G41080 (Arabidopsis thaliana)
Edited Gene(s): atp1
Editing Type(s): C-to-U (3)
Publication(s): [1] Identification of new pentatricopeptide repeat proteins, MREF1 and 2, involved in mitochondrial RNA editing, using computational target RNA prediction., Plant biotechnology (Tokyo, Japan), 2020. [PMID=32362757]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G41080 Mitochondrion atp1 1292 CDS NA NA NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColumbiaWTControlControlControlNASeedling2 weeksSanger sequencing100.00%CompleteNone32362757
SALK_087125CSALK_087125CT-DNA insertionThe T-DNA insertion was located at the vicinity of the stop codon in coding regionsHomozygousTwo-week-old seedlings of the gene-deficient lines displayed no visible aberrant phenotypes under these experimental conditions.Seedling2 weeksSanger sequencing42.11%MediumDecreased32362757
SALK_087125CComplementedComplementationPPR gene-deficient lines were transformed using the floral dip method, with a construct that expresses the corresponding wild-type PPR protein under the control of the A. thaliana ubiquitin-10 promoteHomozygousNASeedling2 weeksSanger sequencing100.00%CompleteRestored32362757
Last update: Feb 2026 (version 2.0)