OCP3 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

1255

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	None	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

149

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	None	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

467

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	High-resolution melting (HRM)	NA	Medium	Decreased	24204264
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Sequencing of individual cDNA clones	64.00%	High	Decreased	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	High-resolution melting (HRM)	NA	High	None	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Sequencing of individual cDNA clones	81.00%	High	None	24204264
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

586

CDS

C-to-U

CAU=>UAU

H=>Y

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Poisoned Primer Extension (PPE) Assay	72.00%	High	Similar	24204264
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Sequencing of individual cDNA clones	77.00%	High	Similar	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	88.00%	High	None	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Sequencing of individual cDNA clones	86.00%	High	None	24204264
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264
Col-0	P. cucumerina inoculation (24h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 24h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

746

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Poisoned Primer Extension (PPE) Assay	89.00%	High	Similar	24204264
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Sequencing of individual cDNA clones	71.00%	High	Similar	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	95.00%	High	None	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Sequencing of individual cDNA clones	92.00%	High	None	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Sequencing of individual cDNA clones	94.00%	High	None	24204264
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	Decreased	24204264
Col-0	P. cucumerina inoculation (24h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 24h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

830

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	High	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264
Col-0	P. cucumerina inoculation (24h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 24h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

836

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Poisoned Primer Extension (PPE) Assay	55.00%	Medium	Decreased	24204264
NA	ocp3	T-DNA Insertion	NA	Homozygous	Impaired in NDH activity, but chloroplast NDH activity-defective mutants show enhanced disease resistance	Leaf	NA	Sequencing of individual cDNA clones	61.00%	High	Decreased	24204264
Col-0	Col-0	Control	Control	Control	Normal	Leaf	NA	Poisoned Primer Extension (PPE) Assay	72.00%	High	None	24204264
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264
Col-0	P. cucumerina inoculation (24h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 24h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhB

872

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264
Col-0	P. cucumerina inoculation (24h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 24h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264

Arabidopsis thaliana

AT5G11270

Chloroplast

ndhD

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	P. cucumerina inoculation (0h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 0h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	None	24204264
Col-0	P. cucumerina inoculation (12h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 12h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Similar	24204264
Col-0	P. cucumerina inoculation (24h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 24h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Medium	Decreased	24204264
Col-0	P. cucumerina inoculation (48h)	Inoculation	Col-0 plants are Inoculated with the fungal pathogen P. cucumerina for 48h	NA	NA	Leaf	5 weeks	RT-PCR bulk sequencing	NA	Low	Decreased	24204264

Editing Factor:	OCP3
Synonym:	OVEREXPRESSOR OF CATIONIC PEROXIDASE 3
Description:	Encoding a homeodomain transcription factor involved in mediating resistance to infection by necrotrophic pathogens dependent on perception of jasmonic acid through COI1; It is expressed in the nucleus, involved in drought tolerance and downregulated upon fungal infection
Protein Family:	NA
Subclass:	NA
Construct Structure:	NA
Gene ID & Species:	AT5G11270 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD
Editing Type(s):	C-to-U (48)
Publication(s):	[1] Mediated plastid RNA editing in plant immunity., PLoS pathogens, 2013. [PMID=24204264]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details