OTP71 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: OTP71
Synonym: ORGANELLE TRANSCRIPT PROCESSING 71
Description: Encoding a pentatricopeptide repeat (PPR) protein involved in RNA editing in mitochondria
Protein Family: PPR
Subclass: E
Physical Interaction: NA
Construct Structure: PLS-E
Gene ID & Species: AT1G64310 (Arabidopsis thaliana)
Edited Gene(s): ccmFn2
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The E domains of pentatricopeptide repeat proteins from different organelles are not functionally equivalent for RNA editing., The Plant journal : for cell and molecular biology, 2013. [PMID=23521509]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G64310 Mitochondrion ccmFn2 176 CDS EnsemblGenomes (release 39)
NA
BK010421
NA
NA NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq75.00%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract92.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighSimilar28761003
Arabidopsis thaliana AT1G64310 Mitochondrion ccmFn2 NA CDS Arabidopsis Genome Initiative NC_001284.2 NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormal growthLeaf15 daysPoisoned primer extension (PPE) assay100.00%CompleteNone23521509
SALK_069611otp71-1T-DNA insertionInsertion site in chromosome 123866165HomozygousNormal growthLeaf15 daysPoisoned primer extension (PPE) assay0.00%UneditedAbsent23521509
SALK_092788otp71-2T-DNA insertionInsertion site in chromosome 123866884HomozygousNormal growthLeaf15 daysPoisoned primer extension (PPE) assay0.00%UneditedAbsent23521509
Last update: Feb 2026 (version 2.0)