Plant Editosome Database
a curated database of RNA editosome in plants

OTP71 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: OTP71
Synonym: ORGANELLE TRANSCRIPT PROCESSING 71
Description: Encodes a pentatricopeptide repeat (PPR) protein involved in RNA editing in mitochondria
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: At1g64310 (Arabidopsis thaliana)
Edited Gene(s): ccmFN2
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The E domains of pentatricopeptide repeat proteins from different organelles are not functionally equivalent for RNA editing, Plant J, 2013. [PMID=23521509]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At1g64310 Mitochondrion ccmFN2 null CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatment No treatmentNo treatmentNormal growthLeaf15 daysPoisoned primer extension (PPE) assay100.00%CompleteNone23521509
SALK_069611otp71-1T-DNA insertionInsertion site in chromosome 123866165HomozygousNormal growthLeaf15 daysPoisoned primer extension (PPE) assay0.00%UneditedAbsent23521509
SALK_092788otp71-2T-DNA insertionInsertion site in chromosome 123866884HomozygousNormal growthLeaf15 daysPoisoned primer extension (PPE) assay0.00%UneditedAbsent23521509
Arabidopsis thaliana At1g64310 Mitochondrion ccmFn2 176 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq75.00%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract92.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)