OTP80 - Plant Editosome Database

Editing Factor:	OTP80
Synonym:	ORGANELLE TRANSCRIPT PROCESSING 80
Description:	Encodes a chloroplast RNA editing factor
Protein Family:	PPR
Subclass:	E,E+
Construct Structure:	PLS-E, NA
Gene ID & Species:	At5g59200 (Arabidopsis thaliana)
Edited Gene(s):	rpl23
Editing Type(s):	C-to-U (9)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] Quantitative analysis of motifs contributing to the interaction between PLS-subfamily members and their target RNA sequences in plastid RNA editing, Plant J, 2014. [PMID=25279799] [4] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites, Plant Cell, 2009. [PMID=19934379]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

At5g59200

Chloroplast
Plastid

null

CDS
NA

C-to-U

NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	No treatment	No treatment	No treatment	Normal growth	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	NA	NA	None	19934379
SALK_111721	otp80-1	T-DNA insertion	The T-DNA insertion is at +141 in CDS	Homozygous	Normal growth	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	0.00%	Unedited	Absent	19934379
SALK_060533	otp80-2	T-DNA insertion	The T-DNA insertion is at +221 in CDS	Homozygous	Normal growth	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	0.00%	Unedited	Absent	19934379
NA	Complement	Complement	The 1870 bp fragments containing the coding sequence of OTP80 was amplified by PCR on total cellular DNA. The construct were cloned into pGWB1 binary vectors and introduced into otp mutants via Agroba	Homozygous	NA	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	NA	NA	Restored	19934379
NA	NA	NA	NA	No treatment	NA	NA	NA	NA	NA	NA	NA	25279799

Arabidopsis thaliana

At5g59200

Plastid
Chloroplast

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	74.09%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	84.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	41.00%	Medium	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	36.00%	Low	Decreased	28761003