OTP80 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: OTP80
Synonym: ORGANELLE TRANSCRIPT PROCESSING 80
Description: OTP80, OTP81, OTP85, and OTP86 target only one editing site each, OTP82 two sites, and OTP84 three sites in different transcripts; An analysis of the target sites requiring the five editing factors involved in editing of multiple sites (CRR22, CRR28, CLB19, OTP82, and OTP84) suggests that editing factors can generally distinguish pyrimidines from purines and, at some positions, must be able to recognize specific bases
Protein Family: PPR
Subclass: E,E+
Construct Structure: PLS-E, NA
Gene ID & Species: AT5G59200 (Arabidopsis thaliana)
Edited Gene(s): rpl23
Editing Type(s): C-to-U (8)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites., The Plant cell, 2009. [PMID=19934379]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G59200 Plastid
Chloroplast
rpl23 89 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq74.09%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract84.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract41.00%MediumDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract36.00%LowDecreased28761003
Arabidopsis thaliana AT5G59200 Chloroplast rpl23 NA CDS C-to-U NA=>NA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0ControlControlControlNormal growthNANART-PCR products are sequencedNANANone19934379
SALK_111721otp80-1T-DNA insertionThe T-DNA insertion is at +141 in CDSHomozygousNormal growthNANART-PCR products are sequenced0.00%UneditedAbsent19934379
SALK_060533otp80-2T-DNA insertionThe T-DNA insertion is at +221 in CDSHomozygousNormal growthNANART-PCR products are sequenced0.00%UneditedAbsent19934379
NAComplementComplementThe 1870 bp fragments containing the coding sequence of OTP80 was amplified by PCR on total cellular DNA. The construct were cloned into pGWB1 binary vectors and introduced into otp mutants via AgrobaHomozygousNANANART-PCR products are sequencedNANARestored19934379
Last update: Jul 2021 (version 1.0)