OTP81 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: OTP81
Synonym: ORGANELLE TRANSCRIPT PROCESSING 81
Description: Encodes a chloroplast RNA editing factor
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: At2g29760 (Arabidopsis thaliana)
Edited Gene(s): rps12
Editing Type(s): C-to-U (4)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites, Plant Cell, 2009. [PMID=19934379]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At2g29760 Chloroplast rps12 null Intronic C-to-U NA=>NA Intronic=>Intronic Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatment No treatmentNo treatmentNormal growthNANART-PCR and poisoned primer extension of RT-PCR products is sNANANone19934379
SALK_092402otp81T-DNA insertionThe T-DNA insertion is at -15 in 5′UTRHomozygousNormal growthNANART-PCR and poisoned primer extension of RT-PCR products is s2.00%PoorDecreased19934379
SALK_092402ComplementComplementThe 3138 bp fragments containing the coding sequence of OTP80 was amplified by PCR on total cellular DNA. The construct were cloned into pGWB1 binary vectors and introduced into otp mutants via AgrobaHomozygousNANANART-PCR and poisoned primer extension of RT-PCR products is sNANARestored19934379
Arabidopsis thaliana At2g29760 Plastid rps12 -69553 Intronic C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq25.74%LowNone32098170
Last update: Jul 2021 (version 1.0)