OTP85 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: OTP85
Synonym: ORGANELLE TRANSCRIPT PROCESSING 85
Description: Encodes a chloroplast RNA editing factor
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-DYW
Gene ID & Species: At2g02980 (Arabidopsis thaliana)
Edited Gene(s): ndhD
Editing Type(s): C-to-U (8)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Quantitative analysis of motifs contributing to the interaction between PLS-subfamily members and their target RNA sequences in plastid RNA editing, Plant J, 2014. [PMID=25279799]
[4] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites, Plant Cell, 2009. [PMID=19934379]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At2g02980 Chloroplast
Plastid
ndhD null CDS
NA
C-to-U NA=>NA S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatment No treatmentNo treatmentNormal growthNANART-PCR and poisoned primer extension of RT-PCR products is sNANANone19934379
SAIL_544_B03otp85T-DNA insertionThe T-DNA insertion is at +1328 in CDSHomozygousNormal growthNANART-PCR and poisoned primer extension of RT-PCR products is s0.00%UneditedAbsent19934379
SAIL_544_B03ComplementComplementThe 2278 bp fragments containing the coding sequence of OTP80 was amplified by PCR on total cellular DNA. The construct were cloned into pGWB1 binary vectors and introduced into otp mutants via AgrobaHomozygousNANANART-PCR and poisoned primer extension of RT-PCR products is sNANARestored19934379
NANANANANo treatmentNANANANANANANA25279799
Arabidopsis thaliana At2g02980 Plastid
Chloroplast
ndhD 674 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq90.13%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract92.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)