OTP87 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: OTP87
Synonym: ORGANELLE TRANSCRIPT PROCESSING 87
Description: Encoding the pentatricopeptide repeat protein OTP87 essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: AT1G74600 (Arabidopsis thaliana)
Edited Gene(s): nad7    atp1
Editing Type(s): C-to-U (14)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The pentatricopeptide repeat protein OTP87 is essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria., The Journal of biological chemistry, 2011. [PMID=21504904]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G74600 Mitochondrion atp1 1178 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GABI_073C06otp87T-DNA InsertionT-DNA is inserted in the coding region( 710 from the ATG start codon)Knockout; HomozygousSmall plants with growth and developmental delaysLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent21504904
GABI_073C06otp87_CPComplementationNANARestores the wild-type phenotype and the expression of OTP87LeafNADirect Sequencing of PCR Products100.00%CompleteRestored21504904
Col-0WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteSimilar21504904
Col-0WTControlControlControlNANANARNA-seq99.24%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT1G74600 Mitochondrion nad7 24 CDS C-to-U AUC=>AUU
NA=>NA
I=>I
NA=>NA
Synonymous
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GABI_073C06otp87T-DNA InsertionT-DNA is inserted in the coding region( 710 from the ATG start codon)Knockout; HomozygousSmall plants with growth and developmental delaysLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent21504904
GABI_073C06otp87_CPComplementationNANARestores the wild-type phenotype and the expression of OTP87LeafNADirect Sequencing of PCR Products100.00%CompleteRestored21504904
Col-0WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteSimilar21504904
Col-0WTControlControlControlNANANARNA-seq97.81%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)