OTP87 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: OTP87
Synonym: ORGANELLE TRANSCRIPT PROCESSING 87
Description: Encoding the pentatricopeptide repeat protein OTP87 essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria.
Protein Family: PPR
Subclass: E
Physical Interaction: NA
Construct Structure: PLS-E
Gene ID & Species: AT1G74600 (Arabidopsis thaliana)
Edited Gene(s): nad7    atp1
Editing Type(s): C-to-U (14)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The pentatricopeptide repeat protein OTP87 is essential for RNA editing of nad7 and atp1 transcripts in Arabidopsis mitochondria., The Journal of biological chemistry, 2011. [PMID=21504904]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G74600 Mitochondrion atp1 1178 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GABI_073C06otp87T-DNA InsertionT-DNA is inserted in the coding region( 710 from the ATG start codon)Knockout; HomozygousSmall plants with growth and developmental delaysLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent21504904
GABI_073C06otp87_CPComplementationNANARestores the wild-type phenotype and the expression of OTP87LeafNADirect Sequencing of PCR Products100.00%CompleteRestored21504904
Col-0WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteSimilar21504904
Col-0WTControlControlControlNANANARNA-seq99.24%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT1G74600 Mitochondrion nad7 24 CDS NA
EnsemblGenomes (release 39)
NA
BK010421
NA NA C-to-U AUC=>AUU
NA=>NA
I=>I
NA=>NA
Synonymous
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GABI_073C06otp87T-DNA InsertionT-DNA is inserted in the coding region( 710 from the ATG start codon)Knockout; HomozygousSmall plants with growth and developmental delaysLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent21504904
GABI_073C06otp87_CPComplementationNANARestores the wild-type phenotype and the expression of OTP87LeafNADirect Sequencing of PCR Products100.00%CompleteRestored21504904
Col-0WTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteSimilar21504904
Col-0WTControlControlControlNANANARNA-seq97.81%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Feb 2026 (version 2.0)