OTP970 - Plant Editosome Database - CNCB-NGDC

OTP970 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	OTP970
Synonym:	ORGANELLE TRANSCRIPT PROCESSING 970
Description:	A loss-of-function otp970 mutant was impaired in RNA editing of ndhB transcripts at site 149 (ndhB-C149); RNA-immunoprecipitation analysis indicated that OTP970 was associated with the ndhB-C149 site. The complementation of the otp970 mutant with OTP970 lacking the DYW domain failed to restore the RNA editing of ndhB-C149; ndhB gene encodes the B subunit of the NADH dehydrogenase-like (NDH) complex; however, neither NDH activity and stability nor NDH-PSI supercomplex formation were affected in otp970 mutant compared to the wild type, indicating that alteration in amino acid sequence is not necessary for NdhB function
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	AT1G18485 (Arabidopsis thaliana)
Edited Gene(s):	ndhB
Editing Type(s):	C-to-U (11)
Publication(s):	[1] OTP970 Is Required for RNA Editing of Chloroplast Transcripts in ., Genes, 2022. [PMID=35052479]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G18485

Chloroplast

149

CDS

NA

NA

NA

NA

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	NA	3–4-week-old	Sequencing chromatograms of the RT-PCR product	100.00%	Complete	None	35052479
SALK_150217	otp970	T-DNA insertion	The T-DNA insertion mutant was obtained from the Arabidopsis Biological Resource Center, OH, USA.	Homozygous; Knockout	Normal	NA	3–4-week-old	Sequencing chromatograms of the RT-PCR product	0.00%	Unedited	Absent	35052479
SALK_150217	otp970+35S:OTP97012#	Plant transformation	The wild-type At1g18485 genomic fragment was PCR-amplified using genespecific primers and was cloned into the pCAMBIA1301 binary vector, expressing OTP970 from the 35S promoter.	Homozygous; Knockout	Normal	NA	3–4-week-old	Sequencing chromatograms of the RT-PCR product	70.00%	High	Restored	35052479
SALK_150217	otp970+35S:OTP970109#	Plant transformation	The wild-type At1g18485 genomic fragment was PCR-amplified using genespecific primers and was cloned into the pCAMBIA1301 binary vector, expressing OTP970 from the 35S promoter.	Homozygous; Knockout	Normal	NA	3–4-week-old	Sequencing chromatograms of the RT-PCR product	80.00%	High	Decreased	35052479
SALK_150217	otp970+35S:OTP970∆DYW6#	Plant transformation	Otp970 mutant transformed with OTP970 lacking the DYW motif.	Homozygous; Knockout	Normal	NA	3–4-week-old	Sequencing chromatograms of the RT-PCR product	0.00%	Unedited	Decreased	35052479
SALK_150217	otp970+35S:OTP970∆DYW102#	Plant transformation	Otp970 mutant transformed with OTP970 lacking the DYW motif.	Homozygous; Knockout	Normal	NA	3–4-week-old	Sequencing chromatograms of the RT-PCR product	0.00%	Unedited	Decreased	35052479
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Root	The reproductive phase	Sequencing chromatograms of the RT-PCR product	10.00%	Poor	None	35052479
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Stem	The reproductive phase	Sequencing chromatograms of the RT-PCR product	100.00%	Complete	None	35052479
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Rosette	The reproductive phase	Sequencing chromatograms of the RT-PCR product	100.00%	Complete	None	35052479
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Flower	The reproductive phase	Sequencing chromatograms of the RT-PCR product	100.00%	Complete	None	35052479
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Silique	The reproductive phase	Sequencing chromatograms of the RT-PCR product	100.00%	Complete	None	35052479

Last update: Feb 2026 (version 2.0)