PGL1 - Plant Editosome Database - BIG Data Center

PGL1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	PGL1
Synonym:	OsPGL1
Description:	The loss of function of OsPGL1 resulted in defects in both chloroplast RNA editing of ndhD-878 and mitochondrial RNA editing of ccmFc-543, both of which could be restored in transgenic complementation lines; Despite synonymous editing of ccmFc-543, the loss of editing of ndhD-878 caused a failed conversion of serine to leucine, leading to chloroplast dysfunction and defects in the photosynthetic complex; the results of additional experiments demonstrated that OsPGL1 directly binds to both transcripts; Interactions between three OsMORFs (OsMORF2/8/9) and OsPGL1 both in vitro and in vivo were confirmed, implying that OsPGL1 functions in RNA editing via an editosome
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	Os12g06650 (Oryza sativa)
Edited Gene(s):	ndhD ccmFc
Editing Type(s):	C-to-U (8)
Publication(s):	[1] A rice dual-localized pentatricopeptide repeat protein is involved in organellar RNA editing together with OsMORFs., Journal of experimental botany, 2018. [PMID=29562289]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

Os12g06650

Mitochondrion

543

CDS

C-to-U

GUC=>GUU

V=>V

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of the RT-PCR Products	100.00%	Complete	None	29562289
Zhonghua 11	Ospgl1-1	Knockout	A 41 bp deletion from position 288 to 328 in this PPR gene	Heterozygous; Knockout	Pale green leaves at all vegetative stages	Leaf	NA	Direct Sequencing of the RT-PCR Products	0.00%	Unedited	Absent	29562289
Zhonghua 11	Ospgl1-2	Knockout	A 1 bp deletion at position 530 in this PPR gene	Heterozygous; Knockout	Pale green leaves at all vegetative stages	Leaf	NA	Direct Sequencing of the RT-PCR Products	8.00%	Poor	Decreased	29562289
Zhonghua 11	complemented T0 (com)	Knockout;Complement	A 41 bp deletion from position 288 to 328 in this PPR gene;The full-length coding sequence of OsPGL1 driven by the CaMV 35S promoter was inserted into a pCAMBIA-2300 vector, after which the construct	Heterozygous; Knockout	Similar with WT(Completely rescued the mutant pale green leaf phenotype)	Leaf	NA	Direct Sequencing of the RT-PCR Products	100.00%	Complete	Restored	29562289

Oryza sativa

Os12g06650

Chloroplast

878

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Zhonghua 11	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of the RT-PCR Products	100.00%	Complete	None	29562289
Zhonghua 11	Ospgl1-1	Knockout	A 41 bp deletion from position 288 to 328 in this PPR gene	Heterozygous; Knockout	Pale green leaves at all vegetative stages	Leaf	NA	Direct Sequencing of the RT-PCR Products	0.00%	Unedited	Absent	29562289
Zhonghua 11	Ospgl1-2	Knockout	A 1 bp deletion at position 530 in this PPR gene	Heterozygous; Knockout	Pale green leaves at all vegetative stages	Leaf	NA	Direct Sequencing of the RT-PCR Products	0.00%	Unedited	Absent	29562289
Zhonghua 11	complemented T0 (com)	Knockout;Complement	A 41 bp deletion from position 288 to 328 in this PPR gene;The full-length coding sequence of OsPGL1 driven by the CaMV 35S promoter was inserted into a pCAMBIA-2300 vector, after which the construct	Heterozygous; Knockout	Similar with WT(Completely rescued the mutant pale green leaf phenotype)	Leaf	NA	Direct Sequencing of the RT-PCR Products	100.00%	Complete	Restored	29562289

Last update: Jul 2021 (version 1.0)