Plant Editosome Database
a curated database of RNA editosome in plants

PPR035 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: PPR035
Synonym: NA
Description: PPR035 regulated RNA editing at rps4-926 and orfX-406; The defectiveness in RNA editing at these sites had no apparent penalties in rice respiration and vegetative growth
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: LOC_Os01g46230 (Oryza sativa)
Edited Gene(s): rps4    orfX
Editing Type(s): C-to-U (8)
Publication(s): [1] Pentatricopeptide Repeat Gene-Mediated Mitochondrial RNA Editing Impacts on Rice Drought Tolerance., Frontiers in plant science, 2022. [PMID=35928709]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_Os01g46230 Mitochondrion orfX 406 CDS C-to-U NA=>NA R=>W Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Geng, japonica, Upland and lowland riceWTControlControlNo mutantNormalSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing91.95%HighNone35928709
Geng, japonica, Upland and lowland riceppr035-1Knockout mutantUsing the CRISPR-Cas9 method, we create independent stop-gain mutants. In ppr035 mutants, we detected defects in RNA editing at one nonsynonymous editing site at orfX (SNV01178 for orfX-406).KnockoutEnhanced drought- and salt toleranceSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing0.00%UneditedAbsent35928709
Geng, japonica, Upland and lowland riceppr035-2Knockout mutantUsing the CRISPR-Cas9 method, we create independent stop-gain mutants. In ppr035 mutants, we detected defects in RNA editing at one nonsynonymous editing site at orfX (SNV01178 for orfX-406).KnockoutEnhanced drought- and salt toleranceSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing0.00%UneditedAbsent35928709
Geng, japonica, Upland and lowland riceppr035-3Knockout mutantUsing the CRISPR-Cas9 method, we create independent stop-gain mutants. In ppr035 mutants, we detected defects in RNA editing at one nonsynonymous editing site at orfX (SNV01178 for orfX-406).KnockoutEnhanced drought- and salt toleranceSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing0.00%UneditedAbsent35928709
Oryza sativa LOC_Os01g46230 Mitochondrion rps4 926 CDS C-to-U NA=>NA NA=>NA Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Geng, japonica, Upland and lowland riceWTControlControlNo mutantNormalSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing92.57%HighNone35928709
Geng, japonica, Upland and lowland riceppr035-1Knockout mutantUsing the CRISPR-Cas9 method, we create independent stop-gain mutants. In ppr035 mutants, we detected defects in RNA editing at one nonsynonymous editing site at rps4 (SNV00404 for rps4-926).KnockoutEnhanced drought- and salt toleranceSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing0.00%UneditedAbsent35928709
Geng, japonica, Upland and lowland riceppr035-2Knockout mutantUsing the CRISPR-Cas9 method, we create independent stop-gain mutants. In ppr035 mutants, we detected defects in RNA editing at one nonsynonymous editing site at rps4 (SNV00404 for rps4-926).KnockoutEnhanced drought- and salt toleranceSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing0.00%UneditedAbsent35928709
Geng, japonica, Upland and lowland riceppr035-3Knockout mutantUsing the CRISPR-Cas9 method, we create independent stop-gain mutants. In ppr035 mutants, we detected defects in RNA editing at one nonsynonymous editing site at rps4 (SNV00404 for rps4-926).KnockoutEnhanced drought- and salt toleranceSeedling15-day-oldHigh-throughput sequencing and Sanger sequencing0.00%UneditedAbsent35928709
Last update: Jul 2021 (version 1.0)