PPR1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

137

CDS

TAIR10

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

148

CDS

TAIR10

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

149

CDS

TAIR10

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

164

CDS

TAIR10

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

172

CDS

TAIR10

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

424

CDS

TAIR10

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

428

CDS

TAIR10

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

475

CDS

TAIR10

C-to-U

CCA=>UCA

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

476

CDS

TAIR10

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

511

CDS

TAIR10

U-to-C

UCU=>CCU

S=>P

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

512

CDS

TAIR10

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

514

CDS

TAIR10

C-to-U

CGU=>UGU

R=>C

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

554

CDS

TAIR10

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Arabidopsis thaliana

AT1G06580

Mitochondrion

ccmB

CDS

TAIR10

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia (Col-0)	WT	Control	Control	No mutant	Normal	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	ppr1	A T-DNA insertion mutant	NA	Homozygous	Decrease in root growth	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968
SALK_022879C	Comp #9	Complementation	For the complementation assay, the region from -2110 to -1 of PPR1 and the PPR1 full-length cDNA were cloned into pBI121. These constructs were introduced into Agrobacterium tumefaciens strain GV3101	Homozygous	NA	Seedling	7-d-old	Sequencing RT-PCR products	NA	NA	NA	37547968

Editing Factor:	PPR1
Synonym:	Pentatricopeptide Repeat Protein 1
Description:	Under Cd treatments, Pentatricopeptide Repeat Protein 1 (PPR1), the target of miR400, acts as a positive regulator by inducing ROS accumulation; Ppr1 mutation affected the Complex III activity in the electron transport chain and RNA editing of the mitochondrial gene ccmB
Protein Family:	PPR
Subclass:	P
Physical Interaction:	NA
Construct Structure:	P
Gene ID & Species:	AT1G06580 (Arabidopsis thaliana)
Edited Gene(s):	ccmB
Editing Type(s):	C-to-U (39) U-to-C (3)
Publication(s):	[1] Intronic microRNA-directed regulation of mitochondrial reactive oxygen species enhances plant stress tolerance in Arabidopsis., The New phytologist, 2023. [PMID=37547968]

Summary

Editing Details