Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| T12404 | T12404 | Control | Control | No mutant | Normal | Leaf (Young) | 30 days after germination | RT-PCR products were directly sequenced | 13.67% | Poor | None | 41133187 |
| T12404 | gret1 | A T-to-C point mutation | A causal T-to-C missense mutation at SL4.0ch03_3101674 in gret1, resulting in a Cys-toArg substitution | Recessive | At 30 DAG, the gret1 young leaves retained only 44% of WT chlorophyll (Chl) levels; Conversely, in fully developed leaves, the photosynthetic pigment content in gret1 was akin to that of WT plants, indicating that the leaf-color phenotype in gret1 is due to reduced photosynthetic pigments during early growth stages; All measured photosynthetic parameters were significantly reduced, with net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr) decreasing by 57%, 24%, and 24%, respectively; the intercellular CO2 concentration (Ci) saw a 30% increase, signifying a decline in photosynthetic efficacy during the early growth stages; At 30 DAG, chloroplasts in gret1 leaves exhibited a morphology largely akin to that of WT; gret1 chloroplasts in immature yellow leaves displayed disorganized membrane structures; the grana lamellae within the stroma were irregularly stacked, and the thylakoids appeared rounded with a blurred structure; Chloroplasts in gret1 leaves showed well-organized thylakoids and stacked grana, resembling the WT | Leaf (Young) | 30 days after germination | RT-PCR products were directly sequenced | 80.67% | High | Increased | 41133187 |
| T12404 | T12404 | Control | Control | No mutant | Normal | Leaf (Mature) | 30 days after germination | RT-PCR products were directly sequenced | 48.00% | Medium | None | 41133187 |
| T12404 | gret1 | A T-to-C point mutation | A causal T-to-C missense mutation at SL4.0ch03_3101674 in gret1, resulting in a Cys-toArg substitution | Recessive | At 30 DAG, the gret1 young leaves retained only 44% of WT chlorophyll (Chl) levels; Conversely, in fully developed leaves, the photosynthetic pigment content in gret1 was akin to that of WT plants, indicating that the leaf-color phenotype in gret1 is due to reduced photosynthetic pigments during early growth stages; All measured photosynthetic parameters were significantly reduced, with net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr) decreasing by 57%, 24%, and 24%, respectively; the intercellular CO2 concentration (Ci) saw a 30% increase, signifying a decline in photosynthetic efficacy during the early growth stages; At 30 DAG, chloroplasts in gret1 leaves exhibited a morphology largely akin to that of WT; gret1 chloroplasts in immature yellow leaves displayed disorganized membrane structures; the grana lamellae within the stroma were irregularly stacked, and the thylakoids appeared rounded with a blurred structure; Chloroplasts in gret1 leaves showed well-organized thylakoids and stacked grana, resembling the WT | Leaf (Mature) | 30 days after germination | RT-PCR products were directly sequenced | 37.00% | Low | Decreased | 41133187 |
| T12404 | OE plant | Overexpression | Engineering an overexpression plasmid containing the full-length WT SlPPR138 coding sequence under the control of the 35S CaMV promoter and introduced it into the gret1 mutant | Recessive | Normal green leaf coloration | Leaf (Young) | 30 days after germination | RT-PCR products were directly sequenced | 58.67% | Medium | None | 41133187 |
| T12404 | SlPPR138CR | Knockout | Knockout plants were generated using CRISPR/Cas9 technology; The process involved designing and synthesizing single-guide RNA (sgRNA) targeting SlPPR138; This sgRNA was then inserted into the psg-Cas9 | Knockout | The SlPPR138-knockout mutants initially displayed yellowing in the leaves before the 8th true leaf, which then transitioned to normal green coloration in later leaves; Plant height was reduced during the early growth stages, although the number of leaves remained unchanged; By 50 DAG, around the 10th leaf stage, the SlPPR138-knockout plants showed normal growth patterns akin to those of WT plants; The f lowering and fruiting characteristics of the SlPPR138-knockout plants were also comparable to WT | Leaf (Young) | 30 days after germination | RT-PCR products were directly sequenced | 92.67% | High | Increased | 41133187 |
| T12404 | T12404 | Control | Control | No mutant | Normal | Leaf (Young) | 60 days after germination | RT-PCR products were directly sequenced | 43.67% | Medium | None | 41133187 |
| T12404 | gret1 | A T-to-C point mutation | A causal T-to-C missense mutation at SL4.0ch03_3101674 in gret1, resulting in a Cys-toArg substitution | Recessive | Once leaf color normalized, gret1 plants began to exhibit growth patterns that closely resembled those of the WT, including typical flowering and fruit-setting behaviors by 60 DAG | Leaf (Young) | 60 days after germination | RT-PCR products were directly sequenced | 56.67% | Medium | Increased | 41133187 |
