PPR6 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: PPR6
Synonym: OsPPR6
Description: In the osppr6 mutants, mRNA levels of plastidic genes transcribed by the plastid-encoded RNA polymerase decreased, while those of genes transcribed by the nuclear-encoded RNA polymerase increased; We further investigated plastidic RNA editing and splicing in the osppr6 mutants and found that the ndhB transcript was mis-edited and the ycf3 transcript was mis-spliced; Therefore, we demonstrate that OsPPR6, a PPR protein, regulates early chloroplast biogenesis and participates in editing of ndhB and splicing of ycf3 transcripts in rice
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: LOC_OS05G49920 (Oryza sativa)
Edited Gene(s): ndhB
Editing Type(s): C-to-U (3)
Publication(s): [1] OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice, Plant Molecular Biology, 2017. [doi:10.1007/s11103-017-0654-0]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_OS05G49920 Chloroplast ndhB 737 CDS Rice Genome Annotation Project (http://rice.uga.edu) NA NA NA C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Japonica rice cultivar Kohishikariosppr6EMS MutantA point mutation at 1103-bp from the start codon of OsPPR6 changes threonine (Thr) to isoleucine (Ile)HeterozygousEarly chloroplast developmental defects leading to albino leaves and seedling deathLeaf3 leaf stageDirect Sequencing of PCR Products30.00%LowDecreased28856519
Japonica rice cultivar KohishikariWTControlControlNo mutantNormalLeaf3 leaf stageDirect Sequencing of PCR ProductsNAHighNone28856519
93-1193-11ControlControlNo mutantNormalLeaf3 leaf stageDirect Sequencing of PCR Products100.00%CompleteNone28856519
Last update: Sep 2026 (version 2.0)