Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Editing reactions assayed with varying ATP concentrations | With 0 mM ATP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 23.33% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying ATP concentrations | With 1 mM ATP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 30.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying ATP concentrations | With 2 mM ATP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 28.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying ATP concentrations | With 5 mM ATP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 26.88% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying Mg++ concentrations | With 0 mM Mg++ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 25.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying Mg++ concentrations | With 1.5 mM Mg++ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 34.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying Mg++ concentrations | With 3 mM Mg++ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 35.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying Mg++ concentrations | With 6 mM Mg++ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 27.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying temperature conditions | 10 ℃ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 0.50% | Poor | None | 31996373 |
| NA | WT | Editing reactions assayed with varying temperature conditions | 16 ℃ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 1.00% | Poor | None | 31996373 |
| NA | WT | Editing reactions assayed with varying temperature conditions | 22 ℃ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 12.00% | Poor | None | 31996373 |
| NA | WT | Editing reactions assayed with varying temperature conditions | 28 ℃ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 24.00% | Low | None | 31996373 |
| NA | WT | Editing reactions assayed with varying temperature conditions | 34 ℃ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 10.00% | Poor | None | 31996373 |
| NA | WT | Editing reactions assayed with varying temperature conditions | 40 ℃ | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 0.50% | Poor | None | 31996373 |
| NA | WT | Protein peaks from rPPR65 purification | Peak A | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 35.00% | Low | None | 31996373 |
| NA | WT | Protein peaks from rPPR66 purification | Peak B | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 60.00% | High | None | 31996373 |
| NA | WT | Protein peaks from rPPR67 purification | Peak C | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 18.00% | Poor | None | 31996373 |
| NA | WT | Addition of zinc chelator | No treatment | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 65.00% | High | None | 31996373 |
| NA | WT | Addition of zinc chelator | Addition of 8 mM 1,10-o-phenantroline (PHEN) | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 30.00% | Low | None | 31996373 |
| NA | WT | Addition of zinc chelator | Addition of 8 mM EDTA | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 36.00% | Low | None | 31996373 |
| NA | WT | Addition of ATP and non-hydrolyzable analog | 0 mM ATP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 53.00% | Medium | None | 31996373 |
| NA | WT | Addition of ATP and non-hydrolyzable analog | 2mM ATP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 64.00% | High | None | 31996373 |
| NA | WT | Addition of ATP and non-hydrolyzable analog | 2mM ADP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 54.00% | Medium | None | 31996373 |
| NA | WT | Addition of ATP and non-hydrolyzable analog | 2mM AMP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 51.00% | Medium | None | 31996373 |
| NA | WT | Addition of ATP and non-hydrolyzable analog | 2mM AMP-PCP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 61.00% | High | None | 31996373 |
| NA | WT | Addition of ATP and non-hydrolyzable analog | 2mM AMP-CP | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 64.00% | High | None | 31996373 |
| NA | WT | Addition of putative transition state inhibitor | No treatment | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 23.00% | Low | None | 31996373 |
| NA | WT | Addition of putative transition state inhibitor | zebularine (ZEB) | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 24.00% | Low | None | 31996373 |
| NA | WT | Addition of putative transition state inhibitor | deazacytidine (AZA) | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 27.00% | Low | None | 31996373 |
| NA | WT | Addition of putative transition state inhibitor | tetrahydrouridine (THU) | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 26.00% | Low | None | 31996373 |
| NA | WT | RNA oligonucleotides with incorporated zebularine (Z) with the sequence shown were added to PPR65 reactions at 1000:1 molar ratio to substrates. | No treatment | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 59.00% | Medium | None | 31996373 |
| NA | WT | RNA oligonucleotides with incorporated zebularine (Z) with the sequence shown were added to PPR65 reactions at 1000:1 molar ratio to substrates. | 3-mer UZA | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 58.00% | Medium | None | 31996373 |
| NA | WT | RNA oligonucleotides with incorporated zebularine (Z) with the sequence shown were added to PPR65 reactions at 1000:1 molar ratio to substrates. | 11-mer AUCCUZAAAAA | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 62.00% | High | None | 31996373 |
| NA | WT | PPR65 [7 μM] catalyzed reactions at a range of RNA concentrations | 0.08 nM | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 39.00% | Low | None | 31996373 |
| NA | WT | PPR65 [7 μM] catalyzed reactions at a range of RNA concentrations | 0.16 nM | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 40.00% | Medium | None | 31996373 |
| NA | WT | PPR65 [7 μM] catalyzed reactions at a range of RNA concentrations | 0.40 nM | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 37.00% | Low | None | 31996373 |
| NA | WT | PPR65 [7 μM] catalyzed reactions at a range of RNA concentrations | 0.80 nM | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 38.00% | Low | None | 31996373 |
| NA | WT | PPR65 [7 μM] catalyzed reactions at a range of RNA concentrations | 1.6 nM | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 36.00% | Low | None | 31996373 |
| NA | WT | PPR65 [7 μM] catalyzed reactions at a range of RNA concentrations | 4.0 nM | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 35.00% | Low | None | 31996373 |
| NA | WT | Editing assays performed in vitro using different ratios of enzyme versus substrate (E/S) | 375:01:00 | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 70.00% | High | None | 31996373 |
| NA | WT | Editing assays performed in vitro using different ratios of enzyme versus substrate (E/S) | 100:01:00 | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 53.00% | Medium | None | 31996373 |
| NA | WT | Editing assays performed in vitro using different ratios of enzyme versus substrate (E/S) | 10:01 | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | 5.00% | Poor | None | 31996373 |
| NA | WT | Editing assays performed in vitro using different ratios of enzyme versus substrate (E/S) | 1:01 | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | NA | NA | None | 31996373 |
| NA | WT | Editing assays performed in vitro using different ratios of enzyme versus substrate (E/S) | 1:10 | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | NA | NA | None | 31996373 |
| NA | WT | Editing assays performed in vitro using different ratios of enzyme versus substrate (E/S) | 0.111111111 | Non-mutant | NA | NA | NA | Poisoned primer extension (PPE) assay | NA | NA | None | 31996373 |