Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| 9522 | WT-cDNA | Control | Control | NA | Normal | Leaf | NA | RT-PCR products were directly sequenced | 100.00% | Complete | None | 40141287 |
| 9522 | ppr674#1-cDNA | CRISPR/Cas9 | Based on the coding sequence of OsPPR674 (LOC_Os08g03676), the guide RNA (gRNAs) targets in OsPPR674 and primers were selected via the E-CRISP Design Tool; Two single-guide RNAs (sgRNAs)—sgRNA-1 (5′-G | Homozygous | Reduced plant height, decreased seed-setting rate, and poor drought tolerance | Leaf | NA | RT-PCR products were directly sequenced | 0.00% | Unedited | Absent | 40141287 |
| 9522 | ppr674#2-cDNA | CRISPR/Cas9 | Based on the coding sequence of OsPPR674 (LOC_Os08g03676), the guide RNA (gRNAs) targets in OsPPR674 and primers were selected via the E-CRISP Design Tool; Two single-guide RNAs (sgRNAs)—sgRNA-1 (5′-G | Homozygous | Reduced plant height, decreased seed-setting rate, and poor drought tolerance | Leaf | NA | RT-PCR products were directly sequenced | 0.00% | Unedited | Absent | 40141287 |