PPR_91 - Plant Editosome Database

Editing Factor:	PPR_91
Synonym:	Phypa_211228
Description:	Disruption of each gene by homologous recombination revealed that PpPPR_56 was involved in RNA editing at the nad3 and nad4 sites, PpPPR_77 at the cox2 and cox3 sites, and PpPPR_91 at the nad5-2 site in the mitochondrial transcripts; The nucleotide sequences surrounding the two editing sites targeted by a single PPR-DYW protein share 42 to 56% of their identities
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E/E+-DYW
Gene ID & Species:	PP1S68_207V6 (Physcomitrium patens)
Edited Gene(s):	nad5
Editing Type(s):	C-to-U (3)
Publication(s):	[1] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts, Plant Cell Physiology, 2010. [doi:10.1093/pcp/pcq142]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Physcomitrium patens

PP1S68_207V6

Mitochondrion

730

CDS

P. patens subsp. patens genome ver. 1.1

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	△91-1	The Nptii Gene Cassette	Restriction sites - Bamhi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	△91-13	The Nptii Gene Cassette	Restriction sites - Bamhi	Knockout	Severe growth retardation	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	WT	Control	Control	No mutant	Normal	Protonema	3 days	Direct Sequencing of PCR Products	100.00%	Complete	None	20837503