Plant Editosome Database
a curated database of RNA editosome in plants

PPS1 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: PPS1
Synonym: NA
Description: It is confirmed that pss1-RNAi line exhibited enhanced sensitivity to salinity and ABA stress at vegetative stage, specifically; PPS1 functioned on ROS signaling network to contribute for the flexibility to abiotic stresses
Protein Family: PPR
Subclass: DYW,NA
Construct Structure: PLS-E-E+-DYW, NA
Gene ID & Species: Os12g36620 (Oryza sativa)
Edited Gene(s): nad3
Editing Type(s): C-to-U (37)
Publication(s): [1] Silencing of rice PPR gene PPS1 exhibited enhanced sensibility to abiotic stress and remarkable accumulation of ROS., Journal of plant physiology, 2020. [PMID=33429329]
[2] Rice PPS1 encodes a DYW motif-containing pentatricopeptide repeat protein required for five consecutive RNA-editing sites of nad3 in mitochondria., The New phytologist, 2018. [PMID=30019754]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa Os12g36620 Mitochondrion nad3 155 CDS C-to-U CCG=>CUG P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZhongHua 11WTControlControlControlNormalCalliNART-PCR products were sequenced directly73.00%HighNone30019754
ZhongHua 11pps1-RNAiRNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly12.00%PoorDecreased30019754
ZhongHua 11T0-4RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly26.92%LowDecreased30019754
ZhongHua 11T0-6RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly14.29%PoorDecreased30019754
ZhongHua 11T0-21RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly21.43%LowDecreased30019754
ZhongHua 11pps1/+ #1A single-base insertion at target site 1pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 1 for Knockout: GGTTCCGGAGATACACGCGAAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
ZhongHua 11pps1/+ #2A four-base deletion at target site 2pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 2 for Knockout: GCCTGGCCTTGTCCGCAAATAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly10.00%PoorDecreased30019754
Oryza sativa Os12g36620 Mitochondrion nad3 172 CDS C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZhongHua 11WTControlControlControlNormalCalliNART-PCR products were sequenced directly75.00%HighNone30019754
ZhongHua 11pps1-RNAiRNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly10.00%PoorDecreased30019754
ZhongHua 11T0-4RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly13.04%PoorDecreased30019754
ZhongHua 11T0-6RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly5.88%PoorDecreased30019754
ZhongHua 11T0-21RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly10.00%PoorDecreased30019754
ZhongHua 11pps1/+ #1A single-base insertion at target site 1pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 1 for Knockout: GGTTCCGGAGATACACGCGAAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
ZhongHua 11pps1/+ #2A four-base deletion at target site 2pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 2 for Knockout: GCCTGGCCTTGTCCGCAAATAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly15.00%PoorDecreased30019754
Oryza sativa Os12g36620 Mitochondrion nad3 173 CDS C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZhongHua 11WTControlControlControlNormalCalliNART-PCR products were sequenced directly80.00%HighNone30019754
ZhongHua 11pps1-RNAiRNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly15.00%PoorDecreased30019754
ZhongHua 11T0-4RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly50.00%MediumDecreased30019754
ZhongHua 11T0-6RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly25.00%LowDecreased30019754
ZhongHua 11T0-21RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly45.83%MediumDecreased30019754
ZhongHua 11pps1/+ #1A single-base insertion at target site 1pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 1 for Knockout: GGTTCCGGAGATACACGCGAAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
ZhongHua 11pps1/+ #2A four-base deletion at target site 2pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 2 for Knockout: GCCTGGCCTTGTCCGCAAATAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly15.00%PoorDecreased30019754
Oryza sativa Os12g36620 Mitochondrion nad3 190 CDS C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZhongHua 11WTControlControlControlNormalCalliNART-PCR products were sequenced directly79.00%HighNone30019754
ZhongHua 11pps1-RNAiRNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly8.00%PoorDecreased30019754
ZhongHua 11T0-4RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly20.00%LowDecreased30019754
ZhongHua 11T0-6RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly13.64%PoorDecreased30019754
ZhongHua 11T0-21RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly8.70%PoorDecreased30019754
ZhongHua 11pps1/+ #1A single-base insertion at target site 1pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 1 for Knockout: GGTTCCGGAGATACACGCGAAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
ZhongHua 11pps1/+ #2A four-base deletion at target site 2pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 2 for Knockout: GCCTGGCCTTGTCCGCAAATAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
Oryza sativa Os12g36620 Mitochondrion nad3 191 CDS C-to-U CCA=>UUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ZhongHua 11WTControlControlControlNormalCalliNART-PCR products were sequenced directly83.00%HighNone30019754
ZhongHua 11pps1-RNAiRNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly10.00%PoorDecreased30019754
ZhongHua 11T0-4RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly33.33%LowDecreased30019754
ZhongHua 11T0-6RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly28.57%LowDecreased30019754
ZhongHua 11T0-21RNAiA 251 bp fragment (ranging from 56 to 306 bp) of LOC_Os12 g36620 cDNA was cloned into the pH7GWIWG(II) vector to construct the RNAi vector. Calli derived from ZhongHua 11 (Oryza sativa. L. Japonica) wKnockdownNACalliNART-PCR products were sequenced directly20.00%LowDecreased30019754
ZhongHua 11pps1/+ #1A single-base insertion at target site 1pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 1 for Knockout: GGTTCCGGAGATACACGCGAAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
ZhongHua 11pps1/+ #2A four-base deletion at target site 2pps1 knockout plants generated using the CRISPR/Cas9 system. Target site 2 for Knockout: GCCTGGCCTTGTCCGCAAATAGGKnockoutGrowing slowly, dwarfing and delayed development in vegetative stages, smaller and shorter anthers, sterile pollen, lower germination on the stigma, shorter panicles and lower seed‐setting rates.CalliNART-PCR products were sequenced directly0.00%UneditedAbsent30019754
Oryza sativa Os12g36620 Mitochondrion nad3 NA CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Zhonghua 11WTWild Type; Abiotic stress treatmentsFor the abiotic stress treatments, seeds from wild-type line were sterilized and placed on half-strength N6 medium containing 0.5 and 10 μM ABA or 100, 200 and 300 mM NaCl and grown 9 days at normal gNo mutantNormalNANANANANANA33429329
Zhonghua 11pps-RNAiRNA interference; Abiotic stress treatmentsFor the abiotic stress treatments, seeds from pss1-RNAi line were sterilized and placed on half-strength N6 medium containing 0.5 and 10 μM ABA or 100, 200 and 300 mM NaCl and grown 9 days at normal gKnockdownThe pps1-RNAi line has enhanced sensitivity to salinity; The pps1-RNAi line has enhanced sensitivity to ABA; Remarkable accumulation of ROS in pps1-RNAi anthersNANANANANANA33429329
Last update: Jul 2021 (version 1.0)