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PpPPR_45 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: PpPPR_45
Synonym: Phypa_156474
Description: Encoding a DYW-subclass pentatricopeptide repeat (PPR) protein which is a chloroplast RNA editing factor in P. patens
Protein Family: PPR
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: PP1S543_6V6 (Physcomitrium patens)
Edited Gene(s): rps14
Editing Type(s): C-to-U (22)
Publication(s): [1] Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts., FEBS letters, 2014. [PMID=25277299]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Physcomitrium patens PP1S543_6V6 Chloroplast rps14 2 CDS Pp1s543_6V6.1 (http://www.cosmoss.org) NA NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GH moss lineGH lineWithout β-estradiolControlControlNormalProtonema4 daysDirect Sequencing of PCR Products65.00%HighNone25277299
GH moss lineGH lineWith β-estradiolControlControlNormalProtonema4 daysDirect Sequencing of PCR Products65.00%HighNone25277299
GH moss line45RNAi #3RNAi without β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products50.00%MediumDecreased25277299
GH moss line45RNAi #3RNAi with β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products16.70%PoorDecreased25277299
GH moss line45RNAi #5RNAi without β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products40.00%MediumDecreased25277299
GH moss line45RNAi #5RNAi with β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products2.00%PoorDecreased25277299
NA45OX#6OverexpressionThe over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoterExpression levels of PpPPR_45 increased considerablyThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products100.00%CompleteDecreased25277299
NA45OX#203OverexpressionThe over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoterExpression levels of PpPPR_45 increased considerablyThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products90.00%HighDecreased25277299
NA45OX#114OverexpressionThe over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoterExpression levels of PpPPR_45 increased considerablyThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products90.00%HighDecreased25277299
NACTTransformationThe empty pOX7WH1 is introducedCTThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products68.00%HighNone25277299
NAWTControlControlControlNormalProtonema4 daysDirect Sequencing of PCR Products65.00%HighNone25277299
Physcomitrium patens PP1S543_6V6 Chloroplast rps14 NA 5'UTR Pp1s543_6V6.1 (http://www.cosmoss.org) NA NA -1 C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GH moss lineGH lineWithout β-estradiolControlControlNormalProtonema4 daysDirect Sequencing of PCR Products8.00%PoorNone25277299
GH moss lineGH lineWith β-estradiolControlControlNormalProtonema4 daysDirect Sequencing of PCR Products2.00%PoorNone25277299
GH moss line45RNAi #3RNAi without β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products4.00%PoorDecreased25277299
GH moss line45RNAi #3RNAi with β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products2.00%PoorSimilar25277299
GH moss line45RNAi #5RNAi without β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products9.00%PoorSimilar25277299
GH moss line45RNAi #5RNAi with β-estradiolAn inducible RNAi construct is introduced into the transgenic GH moss lineKnockdownRetarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 daysProtonema4 daysDirect Sequencing of PCR Products0.00%UneditedAbsent25277299
NA45OX#6OverexpressionThe over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoterExpression levels of PpPPR_45 increased considerablyThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products33.00%LowIncreased25277299
NA45OX#114OverexpressionThe over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoterExpression levels of PpPPR_45 increased considerablyThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products27.00%LowIncreased25277299
NA45OX#203OverexpressionThe over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoterExpression levels of PpPPR_45 increased considerablyThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products13.00%PoorIncreased25277299
NACTTransformationThe empty pOX7WH1 is introducedCTThe protonemata of the 45OX lines grows slower and had fewer gametophores than the wild typeProtonema4 daysDirect Sequencing of PCR Products9.00%PoorNone25277299
NAWTControlControlControlNormalProtonema4 daysDirect Sequencing of PCR Products10.00%PoorNone25277299
Last update: Feb 2026 (version 2.0)