PpPPR_45 - Plant Editosome Database - BIG Data Center

PpPPR_45 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	PpPPR_45
Synonym:	Phypa_156474
Description:	Encodes a DYW-subclass pentatricopeptide repeat (PPR) protein which is a chloroplast RNA editing factor in P. patens.
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	PP1S543_6V6 (Physcomitrella patens)
Edited Gene(s):	rps14
Editing Type(s):	C-to-U (22)
Publication(s):	[1] Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts, FEBS Letters, 2014. [PMID=25277299]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Physcomitrella patens

PP1S543_6V6

Chloroplast

-1

5'UTR

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
GH moss line	GH line	Without β-estradiol	No treatment	No treatment	No treatment	Protonema	4 days	Direct Sequencing of PCR Products	8.00%	Poor	None	25277299
GH moss line	GH line	With β-estradiol	No treatment	No treatment	No treatment	Protonema	4 days	Direct Sequencing of PCR Products	2.00%	Poor	None	25277299
GH moss line	45RNAi #3	RNAi without β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	4.00%	Poor	Decreased	25277299
GH moss line	45RNAi #3	RNAi with β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	2.00%	Poor	Similar	25277299
GH moss line	45RNAi #5	RNAi without β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	9.00%	Poor	Similar	25277299
GH moss line	45RNAi #5	RNAi with β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	25277299
NA	45OX#6	Overexpression	The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter	Expression levels of PpPPR_45 increased considerably	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	33.00%	Low	Increased	25277299
NA	45OX#114	Overexpression	The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter	Expression levels of PpPPR_45 increased considerably	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	27.00%	Low	Increased	25277299
NA	45OX#203	Overexpression	The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter	Expression levels of PpPPR_45 increased considerably	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	13.00%	Poor	Increased	25277299
NA	CT	Transformation	The empty pOX7WH1 is introduced	CT	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	9.00%	Poor	None	25277299
NA	WT	No treatment	No treatment	No treatment	No treatment	Protonema	4 days	Direct Sequencing of PCR Products	10.00%	Poor	None	25277299

Physcomitrella patens

PP1S543_6V6

Chloroplast

2

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
GH moss line	GH line	Without β-estradiol	No treatment	No treatment	No treatment	Protonema	4 days	Direct Sequencing of PCR Products	65.00%	High	None	25277299
GH moss line	GH line	With β-estradiol	No treatment	No treatment	No treatment	Protonema	4 days	Direct Sequencing of PCR Products	65.00%	High	None	25277299
GH moss line	45RNAi #3	RNAi without β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	25277299
GH moss line	45RNAi #3	RNAi with β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	16.70%	Low	Decreased	25277299
GH moss line	45RNAi #5	RNAi without β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	40.00%	Medium	Decreased	25277299
GH moss line	45RNAi #5	RNAi with β-estradiol	An inducible RNAi construct is introduced into the transgenic GH moss line	Knockdown	Retarded growth when treated with b-estradiol; In addition, the pale-green phenotype is observed on the surface of protonemata colonies grown for 30 days	Protonema	4 days	Direct Sequencing of PCR Products	2.00%	Poor	Decreased	25277299
NA	45OX#6	Overexpression	The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter	Expression levels of PpPPR_45 increased considerably	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	100.00%	Complete	Decreased	25277299
NA	45OX#203	Overexpression	The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter	Expression levels of PpPPR_45 increased considerably	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	90.00%	High	Decreased	25277299
NA	45OX#114	Overexpression	The over-expression p45OX plasmid with pOX7WH1 is constructed, which is derived from the over-expression vector pPpMADS2 7113 containing the E7113 promoter	Expression levels of PpPPR_45 increased considerably	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	90.00%	High	Decreased	25277299
NA	CT	Transformation	The empty pOX7WH1 is introduced	CT	The protonemata of the 45OX lines grows slower and had fewer gametophores than the wild type	Protonema	4 days	Direct Sequencing of PCR Products	68.00%	High	None	25277299
NA	WT	No treatment	No treatment	No treatment	No treatment	Protonema	4 days	Direct Sequencing of PCR Products	65.00%	High	None	25277299

Last update: Jul 2021 (version 1.0)