Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | WT | Wild Type | Wild Type | No mutant | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 79.17% | High | None | 29718364 |
| NA | △71 6-11 | Knockout (KO) line | Procedures to obtain moss lines PpPPR_56 KO (Δ56-22) and PpPPR_71 KO (Δ71 6-11) have been previously described (Ohtani et al. 2010, Tasaki et al. 2010). | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71FL | Complementation | Full-length (FL) version of PpPPR_71 was transformed into the respective KO moss background. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 84.09% | High | Restored | 29718364 |
| NA | 71△DYW | Complementation | Truncated version (ΔDYW) of PpPPR_71 was transformed into the respective KO moss background. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71△E | Complementation | Truncated version (ΔE) of PpPPR_71 was transformed into the respective KO moss background. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71PPR+71E/71DYW | Complementation | Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 80.00% | High | Restored | 29718364 |
| NA | 71PPR+71E/45DYW | Complementation | Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71PPR+71E/56DYW | Complementation | Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71PPR+71E/77DYW | Complementation | Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor. | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71M1 | Complementation | To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2 | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71M2 | Complementation | To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2 | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |
| NA | 71M3 | Complementation | To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2 | Knockout | NA | Protonema | 4-day-old | Direct sequencing was performed on the cDNA | 0.00% | Unedited | Absent | 29718364 |