RRD2 - Plant Editosome Database

Editing Factor:	RRD2
Synonym:	Root Redifferentiation Defective 2
Description:	Through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 (rrd1), rrd2, and root initiation defective 4 (rid4), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis; Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration; Defective protein composition of the mitochondrial electron transport chain was found in rrd2 and rid4; Phenocopying of the TDF mutants by mitochondrial respiratory inhibition and reactive oxygen species (ROS) induction, together with its reversal by ROS scavenging, suggested that ROS generation resulting from impaired RNA processing is the primary cause of the extra cell division observed during early LR development in the TDF mutants
Protein Family:	PPR
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	AT1G32415 (Arabidopsis thaliana)
Edited Gene(s):	ccb2 ccb3
Editing Type(s):	C-to-U (4)
Publication(s):	[1] Temperature-dependent fasciation mutants provide a link between mitochondrial RNA processing and lateral root morphogenesis., eLife, 2021. [PMID=33443014]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G32415

Mitochondrion

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Ler	WT	Under high-temperature conditions	Lateral roots (LRs) were induced at 28°C for 12 hr	No mutant	Normal	Root	NA	Sequencing PCR products	50.00%	Medium	None	33443014
Ler	rrd2	Under high-temperature conditions; Temperature-dependent fasciation (TDF) mutants	Lateral roots (LRs) were induced at 28°C for 12 hr	NA	Cyt c levels on a per mitochondrial protein basis were decreased in rrd2 callus cultured at 28°C for 3 days	Root	NA	Sequencing PCR products	0.00%	Unedited	Absent	33443014

Arabidopsis thaliana

AT1G32415

Mitochondrion

575

CDS

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Ler	WT	Under high-temperature conditions	Lateral roots (LRs) were induced at 28°C for 12 hr	No mutant	Normal	Root	NA	Sequencing PCR products	85.07%	High	None	33443014
Ler	rrd2	Under high-temperature conditions; Temperature-dependent fasciation (TDF) mutants	Lateral roots (LRs) were induced at 28°C for 12 hr; Temperature-dependent fasciation (TDF) mutants	NA	Cyt c levels on a per mitochondrial protein basis were decreased in rrd2 callus cultured at 28°C for 3 days	Root	NA	Sequencing PCR products	0.00%	Unedited	Absent	33443014