SLG1 - Plant Editosome Database

Editing Factor:	SLG1
Synonym:	SLOW GROWTH 1
Description:	NADH dehydrogenase activity of complex I in slg1 is strongly impaired and production of ATP is reduced; When responding to ABA treatment, slg1 accumulates more H(2) O(2) in its guard cells than the wild type; The slg1 mutant also has an increased expression of genes involved in the alternative respiratory pathway, which may compensate for the disrupted function of complex I and help scavenge the excess accumulation of H(2) O(2)
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E-E+
Gene ID & Species:	AT5G08490 (Arabidopsis thaliana)
Edited Gene(s):	nad3
Editing Type(s):	C-to-U (5)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis, The Plant Journal, 2012. [doi:10.1111/j.1365-313X.2011.04883.x]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G08490

Mitochondrion

250

CDS

TAIR (http://www.arabidopsis.org/)
NA

C-to-U

CCU=>UCU
NA=>NA

P=>S
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Seedling	8 days	Direct Sequencing of PCR Products	72.00%	High	None	22248025
Col-0	slg1	T-DNA Insertion	The T-DNA is inserted into the gene At5g08490 at 170 bp after the initial codon ATG	Knockout	Slow growth and delayed development, as well as altered responses to various abiotic stresses.	Seedling	8 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22248025
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	86.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	23.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	24.00%	Low	Decreased	28761003