SLO1 - Plant Editosome Database

Summary

Editing Factor:	SLO1
Synonym:	SLOW GROWTH 1
Description:	SLO1 protein may indirectly regulate plant growth and development via affecting mitochondrial RNA editing and gene expression
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT2G22410 (Arabidopsis thaliana)
Edited Gene(s):	nad4 nad9
Editing Type(s):	C-to-U (12)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The SLO1 PPR protein is required for RNA editing at multiple sites with similar upstream sequences in Arabidopsis mitochondria, The Plant Journal, 2010. [doi:10.1111/j.1365-313X.2010.04258.x]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G22410

Mitochondrion

nad4

449

CDS

Y08501
NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	slo1-1	T-DNA Insertion	T-DNA is located at -38 bp	Recessive	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
SALK_071273	slo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	NA	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
Col-0	WT	Control	Control	No mutant	Normal	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	20497377
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	27.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	21.00%	Low	Decreased	28761003

Arabidopsis thaliana

AT2G22410

Mitochondrion

nad9

328

CDS

Y08501
NA

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	slo1-1	T-DNA Insertion	T-DNA is located at -38 bp	Recessive	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
SALK_071273	slo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	NA	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
Col-0	WT	Control	Control	No mutant	Normal	Seedling	2 weeks	Direct Sequencing of PCR Products	81.00%	High	None	20497377
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	20.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	20.00%	Low	Decreased	28761003