SLO1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: SLO1
Synonym: SLOW GROWTH 1
Description: Encodes a PPR protein required for RNA editing at multiple sites, its absence gives rise to small plants with slow growth and delayed development.
Protein Family: PPR
Subclass: E,E+
Construct Structure: PLS-E, NA
Gene ID & Species: AT2G22410 (Arabidopsis thaliana)
Edited Gene(s): nad4    nad9
Editing Type(s): C-to-U (14)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The SLO1 PPR protein is required for RNA editing at multiple sites with similar upstream sequences in Arabidopsis mitochondria, The Plant Journal, 2010. [PMID=20497377]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G22410 Mitochondrion nad4 449 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0slo1-1T-DNA InsertionT-DNA is located at -38 bpRecessive mutant; The SLO1 transcripts were not detectableLate germination, slow growth and delayed development; Small plantSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent20497377
SALK_071273slo1-2T-DNA InsertionT-DNA is inserted in the coding regionThe SLO1 transcripts were not detectableLate germination, slow growth and delayed development; Small plantSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent20497377
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling2 weeksDirect Sequencing of PCR Products100.00%CompleteNone20497377
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq97.37%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract27.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract21.00%LowDecreased28761003
Arabidopsis thaliana AT2G22410 Mitochondrion nad9 328 CDS C-to-U CGG=>UGG
NA=>NA
R=>W
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0slo1-1T-DNA InsertionT-DNA is located at -38 bpRecessive mutant; The SLO1 transcripts were not detectableLate germination, slow growth and delayed development; Small plantSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent20497377
SALK_071273slo1-2T-DNA InsertionT-DNA is inserted in the coding regionThe SLO1 transcripts were not detectableLate germination, slow growth and delayed development; Small plantSeedling2 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent20497377
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling2 weeksDirect Sequencing of PCR Products81.00%HighNone20497377
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.89%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract20.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract20.00%LowDecreased28761003
Last update: Jul 2021 (version 1.0)