SLO1 - Plant Editosome Database

Summary

Editing Factor:	SLO1
Synonym:	SLOW GROWTH 1
Description:	Encodes a PPR protein required for RNA editing at multiple sites, its absence gives rise to small plants with slow growth and delayed development.
Protein Family:	PPR
Subclass:	E,E+
Construct Structure:	PLS-E, NA
Gene ID & Species:	AT2G22410 (Arabidopsis thaliana)
Edited Gene(s):	nad4 nad9
Editing Type(s):	C-to-U (14)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] The SLO1 PPR protein is required for RNA editing at multiple sites with similar upstream sequences in Arabidopsis mitochondria, The Plant Journal, 2010. [PMID=20497377]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G22410

Mitochondrion

nad4

449

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	slo1-1	T-DNA Insertion	T-DNA is located at -38 bp	Recessive mutant; The SLO1 transcripts were not detectable	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
SALK_071273	slo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	The SLO1 transcripts were not detectable	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	20497377
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	97.37%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	27.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	21.00%	Low	Decreased	28761003

Arabidopsis thaliana

AT2G22410

Mitochondrion

nad9

328

CDS

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	slo1-1	T-DNA Insertion	T-DNA is located at -38 bp	Recessive mutant; The SLO1 transcripts were not detectable	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
SALK_071273	slo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	The SLO1 transcripts were not detectable	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Seedling	2 weeks	Direct Sequencing of PCR Products	81.00%	High	None	20497377
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	98.89%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	20.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	20.00%	Low	Decreased	28761003