SLO1 - Plant Editosome Database

Summary

Editing Factor:	SLO1
Synonym:	SLOW GROWTH 1
Description:	Encoding a PPR protein required for RNA editing at multiple sites, its absence gives rise to small plants with slow growth and delayed development.
Protein Family:	PPR
Subclass:	E,E+
Physical Interaction:	NA
Construct Structure:	PLS-E, NA
Gene ID & Species:	AT2G22410 (Arabidopsis thaliana)
Edited Gene(s):	nad4 nad9
Editing Type(s):	C-to-U (14)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] The SLO1 PPR protein is required for RNA editing at multiple sites with similar upstream sequences in Arabidopsis mitochondria., The Plant journal : for cell and molecular biology, 2010. [PMID=20497377]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G22410

Mitochondrion

nad4

449

CDS

NA
EnsemblGenomes (release 39)

Y08501
BK010421
NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	slo1-1	T-DNA Insertion	T-DNA is located at -38 bp	Recessive mutant	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
SALK_071273	slo1-2	T-DNA Insertion	T-DNA is inserted in the coding region	NA	Late germination, slow growth and delayed development; Small plant	Seedling	2 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20497377
Col-0	WT	Control	Control	Control	Normal	Seedling	2 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	20497377
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	97.37%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	27.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	21.00%	Low	Decreased	28761003