SLO2 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G13600

Mitochondrion

mttB

144

CDS

C-to-U

UUC=>UUU
NA=>NA

F=>F
NA=>NA

Synonymous
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	NA	NA	NA	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	30.00%	Low	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Tilling line T94087	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	28.24%	Low	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	37.00%	Low	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

mttB

145

CDS

C-to-U

CCG=>UCG
NA=>NA

P=>S
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	0.00%	Unedited	Absent	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Tilling line T94088	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	90.31%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	94.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

mttB

666

CDS

C-to-U

UCC/UCC=>UCU/UUU
NA=>NA

S=>S/F
NA=>NA

Synonymous/Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	NA	NA	NA	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	50.00%	Medium	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	20.00%	Low	Decreased	22540321
Tilling line T94089	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	10.00%	Poor	Decreased	22540321
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	72.48%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	57.00%	Medium	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	20.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	2.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad1

CDS

C-to-U

ACG=>AUG
NA=>NA

T=>M
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	NA	NA	NA	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	90.00%	High	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	55.00%	Medium	Decreased	22540321
Tilling line T94093	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	65.00%	High	Decreased	22540321
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	4.62%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad1

CDS

C-to-U

CUA=>UUA

L=>L

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	NA	NA	NA	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	25.00%	Low	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22540321
Tilling line T94090	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	50.00%	Medium	Decreased	22540321

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad1

743

CDS

C-to-U

NA=>NA
CCA=>CUA

NA=>NA
P=>L

NA
Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	32.00%	Low	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	7.00%	Poor	Decreased	28761003
Col-0	WT	Control	Control	Control	Normal	Seedling	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	slo2-2	A T-DNA Insertion	A T-DNA insertion at 878 bp downstream of the start codon.	NA	When wild-type seedlings were 3 weeks old, smaller than WT.	Seedling	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003
Col-0	slo2-3	A T-DNA Insertion	A point mutation (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83.	NA	Roots and seedlings were smaller than WT's	Seedling	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	0.00%	Unedited	Absent	28761003
Col-0	Complemented slo2-2	Complemented	Performing complementation experiments using the full-length cDNA fused to GFP driven by its native promoter and the CaMV 35S promoter	NA	Reversal of the delay in leaf emergence and suppressed the reduced growth phenotypes in root and shoot	Seedling	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	50.00%	Medium	Decreased	28761003
Col-0	Complemented slo2-3	Complemented	NA	NA	NA	Seedling	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	75.00%	High	Decreased	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad4L

110

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	0.00%	Unedited	Absent	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Tilling line T94091	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	92.72%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	16.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	5.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad7

739

CDS

C-to-U

CUU=>UUU
NA=>NA

L=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	slo2	NA	NA	NA	Plant growth retardation,delayed development; Sugardependent phenotype	NA	NA	SNaPshot technology	0.00%	Unedited	Absent	22902704
Wassilewskija	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	22540321
SALK_521900	slo2-2	T-DNA Insertion	T-DNA is inserted at 878 bp downstream of the start codon	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Tilling line T94092	slo2-3	EMS Mutant	(C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83	NA	Growth retardation( retarded leaf emergence, restricted root growth, and late flowering)	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22540321
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	96.82%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	6.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	1.00%	Poor	Decreased	28761003

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad9

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	98.04%	High	None	32098170

Editing Factor:	SLO2
Synonym:	SLOW GROWTH 2
Description:	Encoding a mitochondrial PPr rna editing factor which is responsible for 7 editing events in Arabidopsis. Loss-of-function mutation in SLO2 results in plant growth retardation, and delayed development, and leads to the dysfunction of mitochondrial complex I, III and IV.
Protein Family:	PPR
Subclass:	E+
Construct Structure:	PLS-E-E+
Gene ID & Species:	AT2G13600 (Arabidopsis thaliana)
Edited Gene(s):	nad4L mttB nad7 nad1 nad9
Editing Type(s):	C-to-U (60)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] Functional analysis of SLO2 provides new insight into the role of plant PPR proteins., Plant signaling & behavior, 2012. [PMID=22902704] [4] SLO2, a mitochondrial pentatricopeptide repeat protein affecting several RNA editing sites, is required for energy metabolism., The Plant journal : for cell and molecular biology, 2012. [PMID=22540321]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details