SLO4 - Plant Editosome Database

Summary

Editing Factor:	SLO4
Synonym:	SLOW GROWTH 4
Description:	Slo4 mutants were characterized as having restricted root growth, being late flowering and displaying an overall delayed growth phenotype; Protein levels and activity of mitochondrial complex I were decreased and putative complex I assembly intermediates accumulated in the mutant plants; An editing defect, leading to an amino acid change, in the mitochondrial nad4 transcript, encoding for a complex I subunit, was identified; Furthermore, the splicing efficiency of the first intron of nad2, encoding for another complex I subunit, was also decreased
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT4G38010 (Arabidopsis thaliana)
Edited Gene(s):	nad4
Editing Type(s):	C-to-U (6)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1, Plant Molecular Biology, 2017. [doi:10.1007/s11103-016-0566-4]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT4G38010

Mitochondrion

nad4

1033

CDS

C-to-U

CCU=>UCU
NA=>NA

P=>S
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	Seedling	2-3 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	27942959
SAIL_682_E11	slo4-1	T-DNA Insertion	T-DNA is inserted within the first L-type PPR element	Homozygous; Knockout	Having restricted root growth, being late flowering and displaying an overall delayed growth phenotype	Seedling	2-3 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27942959
SALK_062576	slo4-2	T-DNA Insertion	T-DNA is inserted within the second S-type PPR element	Homozygous; Knockout	Having restricted root growth, being late flowering and displaying an overall delayed growth phenotype	Seedling	2-3 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	27942959
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	98.00%	High	Similar	28761003