SMK1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: SMK1
Synonym: Small Kernel1_ ZmPPR134
Description: Encodes a pentatricopeptide repeat protein required for mitochondrial nad7 transcript editing and seed development.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: LOC_OS11G10740 (Oryza sativa)
GRMZM2G03148 (Zea mays subsp. mays)
Edited Gene(s): nad7
Editing Type(s): C-to-U (7)
Publication(s): [1] Small kernel 1 encodes a pentatricopeptide repeat protein required for mitochondrial nad7 transcript editing and seed development in maize (Zea mays) and rice (Oryza sativa), The Plant Journal, 2014. [PMID=24923534]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa LOC_OS11G10740 Mitochondrion nad7 836 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
RMD_04Z11IX21Os_smk1-1T-DNA InsertionT-DNA is inserted at +724 bp downstream of the start codon; The insertion is located in the eighth PPR repeatNAChalky and shrunken seedsLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent24923534
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone24923534
Zea mays subsp. mays GRMZM2G03148 Mitochondrion nad7 836 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAsmk1-1Mu insertionMu is inserted in the 10th PPR repeatKnockoutAbnormal embryo and endosperm development, resulting in embryo or seedling lethalityKernel9 DAPDirect Sequencing of PCR Products0.00%UneditedAbsent24923534
NAsmk1-1Mu insertionMu is inserted in the 10th PPR repeatKnockoutAbnormal embryo and endosperm development, resulting in embryo or seedling lethalityKernel11 DAPDirect Sequencing of PCR Products0.00%UneditedAbsent24923534
NAsmk1-1Mu insertionMu is inserted in the 10th PPR repeatKnockoutAbnormal embryo and endosperm development, resulting in embryo or seedling lethalityKernel13 DAPDirect Sequencing of PCR Products0.00%UneditedAbsent24923534
NAsmk1-1Mu insertionMu is inserted in the 10th PPR repeatKnockoutAbnormal embryo and endosperm development, resulting in embryo or seedling lethalityKernel21 DAPDirect Sequencing of PCR Products0.00%UneditedAbsent24923534
NAWTNo treatmentNo treatmentNo treatmentNo treatmentKernel13 DAPDirect Sequencing of PCR Products100.00%CompleteNone24923534
Last update: Jul 2021 (version 1.0)