SMK1 - Plant Editosome Database

Editing Factor:	SMK1
Synonym:	Small Kernel1
Description:	Encoding a pentatricopeptide repeat protein required for mitochondrial nad8 transcript editing and seed development.
Protein Family:	PPR
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	LOC_OS11G10740 (Oryza sativa) GRMZM2G03148 (Zea mays)
Edited Gene(s):	nad7
Editing Type(s):	C-to-U (7)
Publication(s):	[1] Small kernel 1 encodes a pentatricopeptide repeat protein required for mitochondrial nad7 transcript editing and seed development in maize (Zea mays) and rice (Oryza sativa)., The Plant journal : for cell and molecular biology, 2014. [PMID=24923534]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

LOC_OS11G10740

Mitochondrion

836

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
RMD_04Z11IX21	Os_smk1-1	T-DNA Insertion	T-DNA is inserted at +724 bp downstream of the start codon; The insertion is located in the eighth PPR repeat	NA	Chalky and shrunken seeds	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	None	24923534

Zea mays

GRMZM2G03148

Mitochondrion

836

CDS

B73

NB mitochondrial genome

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	9 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	11 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	13 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	21 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	WT	Control	Control	Control	Normal	Kernel	13 DAP	Direct Sequencing of PCR Products	100.00%	Complete	None	24923534