SMK1 - Plant Editosome Database

Editing Factor:	SMK1
Synonym:	Small Kernel 1
Description:	Loss of SMK1 function abolishes the C-to-U editing at the nad7-836 site, leading to the retention of a proline codon that is edited to encode leucine in the wild type; The smk1 mutant showed dramatically reduced complex-I assembly and NADH dehydrogenase activity, and abnormal biogenesis of the mitochondria; Analysis of the ortholog in Oryza sativa (rice) reveals that rice SMK1 has a conserved function in C-to-U editing of the mitochondrial nad7-836 site; The leucine at NAD7-279 is highly conserved from bacteria to flowering plants, and analysis of genome sequences from many plants revealed a molecular coevolution between the requirement for C-to-U editing at this site and the existence of an SMK1 homolog
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	GRMZM2G03148 (Zea mays)
Edited Gene(s):	nad7
Editing Type(s):	C-to-U (5)
Publication(s):	[1] Small kernel 1 encodes a pentatricopeptide repeat protein required for mitochondrial nad7 transcript editing and seed development in maize (Zea mays) and rice (Oryza sativa), The Plant Journal, 2014. [doi:10.1111/tpj.12584]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G03148

Mitochondrion

836

CDS

B73

NB mitochondrial genome

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	9 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	11 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	13 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	smk1-1	Mu insertion	Mu is inserted in the 10th PPR repeat	Knockout	Abnormal embryo and endosperm development, resulting in embryo or seedling lethality	Kernel	21 DAP	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	24923534
NA	WT	Control	Control	No mutant	Normal	Kernel	13 DAP	Direct Sequencing of PCR Products	100.00%	Complete	None	24923534