SMK6 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G13600

Mitochondrion

mttB

145

CDS

C-to-U

CUC=>UUC

P=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031
SALK_021900C	slo2	Control	Control	No mutant	Slow growth	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Unedited	NA	31234031
SALK_021900C	Smk6-OX-4	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Unedited	NA	31234031
SALK_021900C	Smk6-OX-5	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Unedited	NA	31234031
SALK_021900C	Smk6-OX-8	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Arabidopsis thaliana

AT2G13600

Mitochondrion

mttB

146

CDS

C-to-U

UCG=>CCG

S=>P

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	NA	31234031
SALK_021900C	slo2	Control	Control	No mutant	Slow growth	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Unedited	NA	31234031
SALK_021900C	Smk6-OX-4	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	NA	31234031
SALK_021900C	Smk6-OX-5	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	High	NA	31234031
SALK_021900C	Smk6-OX-8	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad4L

110

CDS

C-to-U

CAA=>UAA

Q=>Stop codon

Nonsense

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	High	NA	31234031
SALK_021900C	slo2	Control	Control	No mutant	Slow growth	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Unedited	NA	31234031
SALK_021900C	Smk6-OX-4	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	High	NA	31234031
SALK_021900C	Smk6-OX-5	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	NA	31234031
SALK_021900C	Smk6-OX-8	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	NA	31234031

Arabidopsis thaliana

AT2G13600

Mitochondrion

nad7

739

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	High	NA	31234031
SALK_021900C	slo2	Control	Control	No mutant	Slow growth	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Unedited	NA	31234031
SALK_021900C	Smk6-OX-4	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	High	NA	31234031
SALK_021900C	Smk6-OX-5	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	NA	31234031
SALK_021900C	Smk6-OX-8	Overexpression	Overexpression	No mutant	Grow well	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Zea mays

GRMZM2G010046

Mitochondrion

mttB

138

CDS

C-to-U

CUC=>UUC

P=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	NA	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	None	31234031
UniformMu	smk6	Mu transposon insertion	Mu transposon insertion	Heterozygous; Recessive	The development of both embryo and endosperm is delayed in the smk6 kernels	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Zea mays

GRMZM2G010046

Mitochondrion

mttB

139

CDS

C-to-U

UCG=>CCG

S=>P

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	NA	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	None	31234031
UniformMu	smk6	Mu transposon insertion	Mu transposon insertion	Heterozygous; Recessive	The development of both embryo and endosperm is delayed in the smk6 kernels	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Zea mays

GRMZM2G010046

Mitochondrion

nad1

740

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	NA	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Medium	None	31234031
UniformMu	smk6	Mu transposon insertion	Mu transposon insertion	Heterozygous; Recessive	The development of both embryo and endosperm is delayed in the smk6 kernels	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Zea mays

GRMZM2G010046

Mitochondrion

nad4L

110

CDS

C-to-U

CAA=>UAA

Q=>Stop codon

Nonsense

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	NA	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	None	31234031
UniformMu	smk6	Mu transposon insertion	Mu transposon insertion	Heterozygous; Recessive	The development of both embryo and endosperm is delayed in the smk6 kernels	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Zea mays

GRMZM2G010046

Mitochondrion

nad7

739

CDS

C-to-U

CUU=>UUU

L=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Control	Control	No mutant	NA	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	High	None	31234031
UniformMu	smk6	Mu transposon insertion	Mu transposon insertion	Heterozygous; Recessive	The development of both embryo and endosperm is delayed in the smk6 kernels	Kernel	11 DAP	Direct Sequencing of PCR Products	NA	Low	NA	31234031

Editing Factor:	SMK6
Synonym:	SMALL KERNEL 6; SLO2; SLOW GROWTH 2
Description:	Loss of Smk6 function affects the C-to-U editing at nad1-740, nad4L-110, nad7-739, and mttB-138,139 sites, impairs mitochondrial activity and blocks embryogenesis and endosperm development; Genetic and molecular analysis indicated that SMK6 is the maize ortholog of the Arabidopsis SLO2, which is a component of the PPR-E+/NVWA/DYW2 editing complex; However, yeast two-hybrid analyses did not detect any interaction between SMK6 and any of the mitochondrion-targeted RIPs/MORFs, suggesting that RIPs/MORFs may not be a component of PPR-E+/NVWA/DYW2 RNA editing complex
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E-E+
Gene ID & Species:	AT2G13600 (Arabidopsis thaliana) GRMZM2G010046 (Zea mays)
Edited Gene(s):	nad1 nad4L nad7 mttB
Editing Type(s):	C-to-U (30)
Publication(s):	[1] SMK6 mediates the C-to-U editing at multiple sites in maize mitochondria, Journal of?Plant?Physiology, 2019. [doi:10.1016/j.jplph.2019.152992]

Summary

Editing Details