Plant Editosome Database
a curated database of RNA editosome in plants

SMK6 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: SMK6
Synonym: SMALL KERNEL6, SLO2
Description: Loss of Smk6 function affects the C-to-U editing at nad1-740, nad4L-110, nad7-739, and mttB-138,139 sites, impairs mitochondrial activity and blocks embryogenesis and endosperm development; Genetic and molecular analysis indicated that SMK6 is the maize ortholog of the Arabidopsis SLO2, which is a component of the PPR-E+/NVWA/DYW2 editing complex; However, yeast two-hybrid analyses did not detect any interaction between SMK6 and any of the mitochondrion-targeted RIPs/MORFs, suggesting that RIPs/MORFs may not be a component of PPR-E+/NVWA/DYW2 RNA editing complex.
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E-E+
Gene ID & Species: AT2G13600 (Arabidopsis thaliana)
GRMZM2G010046 (Zea mays)
Edited Gene(s): nad1    nad4L    nad7    mttB
Editing Type(s): C-to-U (30)
Publication(s): [1] SMK6 mediates the C-to-U editing at multiple sites in maize mitochondria., Journal of plant physiology, 2019. [PMID=31234031]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G13600 Mitochondrion mttB 145 CDS C-to-U CUC=>UUC P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0ControlControlControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
SALK_021900Cslo2ControlControlControlSlow growthKernel11 DAPDirect Sequencing of PCR ProductsNAUneditedNA31234031
SALK_021900CSmk6-OX-4OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAUneditedNA31234031
SALK_021900CSmk6-OX-5OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAUneditedNA31234031
SALK_021900CSmk6-OX-8OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Arabidopsis thaliana AT2G13600 Mitochondrion mttB 146 CDS C-to-U UCG=>CCG S=>P Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0ControlControlControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNA31234031
SALK_021900Cslo2ControlControlControlSlow growthKernel11 DAPDirect Sequencing of PCR ProductsNAUneditedNA31234031
SALK_021900CSmk6-OX-4OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNA31234031
SALK_021900CSmk6-OX-5OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAHighNA31234031
SALK_021900CSmk6-OX-8OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Arabidopsis thaliana AT2G13600 Mitochondrion nad4L 110 CDS C-to-U CAA=>UAA S=>S/L Synonymous/Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0ControlControlControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAHighNA31234031
SALK_021900Cslo2ControlControlControlSlow growthKernel11 DAPDirect Sequencing of PCR ProductsNAUneditedNA31234031
SALK_021900CSmk6-OX-4OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAHighNA31234031
SALK_021900CSmk6-OX-5OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNA31234031
SALK_021900CSmk6-OX-8OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNA31234031
Arabidopsis thaliana AT2G13600 Mitochondrion nad7 739 CDS C-to-U CUU=>UUU P=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0ControlControlControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAHighNA31234031
SALK_021900Cslo2ControlControlControlSlow growthKernel11 DAPDirect Sequencing of PCR ProductsNAUneditedNA31234031
SALK_021900CSmk6-OX-4OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAHighNA31234031
SALK_021900CSmk6-OX-5OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNA31234031
SALK_021900CSmk6-OX-8OverexpressionOverexpressionControlGrow wellKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Zea mays GRMZM2G010046 Mitochondrion mttB 138 CDS C-to-U CUC=>UUC P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAsmk6ControlControlControlNAKernel11 DAPDirect Sequencing of PCR ProductsNALowNone31234031
UniformMusmk6Mu transposon insertion,Mu transposon insertion,NULLThe development of both embryo and endosperm is delayed in the smk6 kernelsKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Zea mays GRMZM2G010046 Mitochondrion mttB 139 CDS C-to-U UCG=>CCG S=>P Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAsmk6ControlControlControlNAKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNone31234031
UniformMusmk6Mu transposon insertion,Mu transposon insertion,NULLThe development of both embryo and endosperm is delayed in the smk6 kernelsKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Zea mays GRMZM2G010046 Mitochondrion nad1 740 CDS C-to-U CCA=>CUA P=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAsmk6ControlControlControlNAKernel11 DAPDirect Sequencing of PCR ProductsNAMediumNone31234031
UniformMusmk6Mu transposon insertion,Mu transposon insertion,Mu transposon insertion,The development of both embryo and endosperm is delayed in the smk6 kernelsKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Zea mays GRMZM2G010046 Mitochondrion nad4L 110 CDS C-to-U CAA=>UAA S=>S/L Synonymous/Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAsmk6ControlControlControlNAKernel11 DAPDirect Sequencing of PCR ProductsNALowNone31234031
UniformMusmk6Mu transposon insertion,Mu transposon insertion,NULLThe development of both embryo and endosperm is delayed in the smk6 kernelsKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Zea mays GRMZM2G010046 Mitochondrion nad7 739 CDS C-to-U CUU=>UUU P=>L Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAsmk6ControlControlControlNAKernel11 DAPDirect Sequencing of PCR ProductsNAHighNone31234031
UniformMusmk6Mu transposon insertion,Mu transposon insertion,NULLThe development of both embryo and endosperm is delayed in the smk6 kernelsKernel11 DAPDirect Sequencing of PCR ProductsNALowNA31234031
Last update: Jul 2021 (version 1.0)