Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Nipponbare | WT | Control | Control | No mutant | Normal | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 96.15% | High | None | 36096762 |
| NA | ssa1 | Deletion | The ssa1 locus was fine mapped to a interval between markers InD2891 and InD2922 on chromosome 2 (Chr. 2). There is a 14 bp deletion on the first exon of the SSA1. | Heterozygous | Albino and seedling-lethal phenotypes. he mutation of SSA1 resulted in a slightly shorter plant height and reduced chlorophyll content. The albino phenotype appeared in the whole seedling after seed germination in ssa1, but the growth was completely normal before the four-leaf stage. Then, the ssa1 plant withered and died at the seedling stage after the four-leaf stage. Transmission electron microscopy (TEM) was used to observe the cytological structure of leaves. The chloroplast structure was almost unaffected in WT, while the chloroplasts collapsed and shrank in ssa1 | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 54.84% | Medium | Decreased | 36096762 |
| NA | Com1 | Complementation | For complementation tests of the ssa1 mutant, the CDS of SSA1 was cloned into the pCAMBIA2300 binary vector and expressed by the driving actin promoter. | Heterozygous | All positive complementary line plants developed normal green leaves at seedling stages and were restored to WT chlorophyll levels. The structure of chloroplasts was also detected by TEM. | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 91.84% | High | Restored | 36096762 |
| NA | Com2 | Complementation | For complementation tests of the ssa1 mutant, the CDS of SSA1 was cloned into the pCAMBIA2300 binary vector and expressed by the driving actin promoter. | Heterozygous | All positive complementary line plants developed normal green leaves at seedling stages and were restored to WT chlorophyll levels. The structure of chloroplasts was also detected by TEM. | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 100.00% | Complete | Restored | 36096762 |
| NA | Com3 | Complementation | For complementation tests of the ssa1 mutant, the CDS of SSA1 was cloned into the pCAMBIA2300 binary vector and expressed by the driving actin promoter. | Heterozygous | All positive complementary line plants developed normal green leaves at seedling stages and were restored to WT chlorophyll levels. The structure of chloroplasts was also detected by TEM. | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 100.00% | Complete | Restored | 36096762 |
| Nipponbare | ssa1-2 | Knockout | To further confirm the result that the phenotype of ssa1 was caused by the functional loss of LOC_Os02g47360, the CRISPR–Cas9 system was used to construct a knockout mutant of this gene. Among the mul | Knockout | Albinism at the seedling stage with reduced chlorophyll content. | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 56.04% | Medium | Decreased | 36096762 |
| Nipponbare | ssa1-9 | Knockout | To further confirm the result that the phenotype of ssa1 was caused by the functional loss of LOC_Os02g47360, the CRISPR–Cas9 system was used to construct a knockout mutant of this gene. Among the mul | Knockout | Albinism at the seedling stage with reduced chlorophyll content. | Seedling | NA | Sequencing chromatograms were derived by direct sequencing o | 50.00% | Medium | Decreased | 36096762 |
