Plant Editosome Database
a curated database of RNA editosome in plants

TAD3 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: TAD3
Synonym: ATTAD3; EMB2820; EMBRYO DEFECTIVE 2820; TRNA ADENOSINE DEAMINASE 3
Description: Encoding a protein belonging to cytidine/deoxycytidylate deaminase family. Knockout of TAD3 is lethal and leads to arrested embryo development at the globular stage.
Protein Family: Adenosine deaminase
Subclass: NA
Construct Structure: NA
Gene ID & Species: AT5G24670 (Arabidopsis thaliana)
Edited Gene(s): tRNA-Ala(AGC)    tRNA-Ser(AGA)    tRNA-Leu(AAG)    tRNA-Ile(AAT)    tRNA-Arg(ACG)    tRNA-Thr(AGT)
Editing Type(s): A-to-I (12)
Publication(s): [1] Identification of enzymes for adenosine-to-inosine editing and discovery of cytidine-to-uridine editing in nucleus-encoded transfer RNAs of Arabidopsis., Plant physiology, 2014. [PMID=25315605]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Ala(AGC) 34 Exon A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAHighDecreased25315605
Col-0WTControlControlControlNormalLeafNADirect sequencing of PCR products100.00%CompleteNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Arg(ACG) 34 Exon A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAMediumDecreased25315605
Col-0WTControlControlControlNormalLeafNADirect sequencing of PCR products100.00%CompleteNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Ile(AAT) 34 Exon A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNALowDecreased25315605
Col-0WTControlControlControlNormalLeafNADirect sequencing of PCR productsNAMediumNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Leu(AAG) 34 Exon A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNALowDecreased25315605
Col-0WTControlControlControlNormalLeafNADirect sequencing of PCR productsNAHighNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Ser(AGA) 34 Exon A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAMediumDecreased25315605
Col-0WTControlControlControlNormalLeafNADirect sequencing of PCR productsNAHighNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Thr(AGT) 34 Exon A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAHighDecreased25315605
Col-0WTControlControlControlNormalLeafNADirect sequencing of PCR products100.00%CompleteNone25315605
Last update: Jul 2021 (version 1.0)