PED
Plant
Editosome
Database
TAD3 - Plant Editosome Database - CNCB-NGDC
  1. Home
  2. Editing Factors
  3. TAD3

Summary

Editing Factor: TAD3
Synonym: AtTAD3; EMB2820; EMBRYO DEFECTIVE 2820; TRNA ADENOSINE DEAMINASE 3
Description: Knockdown mutants for AtTAD2 and AtTAD3 display reduced growth and inefficient editing from adenosine to inosine in six nucleus-encoded tRNA species; Moreover, upon comparison of DNA and complementary DNA sequences, we discovered cytidine-to-uridine RNA editing in position 32 of two nucleus-encoded serine tRNAs, tRNA-serine(AGA) and tRNA-serine(GCT)
Protein Family: Adenosine deaminases acting on tRNA (ADAT)
Subclass: NA
Physical Interaction: TAD2
Construct Structure: NA
Gene ID & Species: AT5G24670 (Arabidopsis thaliana)
Edited Gene(s): tRNA-Ala (AGC)    tRNA-Ser (AGA)    tRNA-Leu (AAG)    tRNA-Ile (AAT)    tRNA-Arg (ACG)    tRNA-Thr (AGT)
Editing Type(s): A-to-I (12)
Publication(s): [1] Identification of Enzymes for Adenosine-to-Inosine Editing and Discovery of Cytidine-to-Uridine Editing in Nucleus-Encoded Transfer RNAs of Arabidopsis, Plant Physiology, 2014. [doi:10.1104/pp.114.250498]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Ala (AGC) 34 Exon TAIR (http://www.arabidopsis.org) NA NA NA A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAHighDecreased25315605
Col-0WTControlControlNo mutantNormalLeafNADirect sequencing of PCR products100.00%CompleteNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Arg (ACG) 34 Exon TAIR (http://www.arabidopsis.org) NA NA NA A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAMediumDecreased25315605
Col-0WTControlControlNo mutantNormalLeafNADirect sequencing of PCR products100.00%CompleteNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Ile (AAT) 34 Exon TAIR (http://www.arabidopsis.org) NA NA NA A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNALowDecreased25315605
Col-0WTControlControlNo mutantNormalLeafNADirect sequencing of PCR productsNAMediumNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Leu (AAG) 34 Exon TAIR (http://www.arabidopsis.org) NA NA NA A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNALowDecreased25315605
Col-0WTControlControlNo mutantNormalLeafNADirect sequencing of PCR productsNAHighNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Ser (AGA) 34 Exon TAIR (http://www.arabidopsis.org) NA NA NA A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAMediumDecreased25315605
Col-0WTControlControlNo mutantNormalLeafNADirect sequencing of PCR productsNAHighNone25315605
Arabidopsis thaliana AT5G24670 Nucleus tRNA-Thr (AGT) 34 Exon TAIR (http://www.arabidopsis.org) NA NA NA A-to-I NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0tad3-RNAi-GRNAiAgrobacterium tumefaciens-mediated transformationKnockdownA mild growth retardationLeafNADirect sequencing of PCR productsNAHighDecreased25315605
Col-0WTControlControlNo mutantNormalLeafNADirect sequencing of PCR products100.00%CompleteNone25315605
Last update: Sep 2026 (version 2.0)