Experiment Details
Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
GK-119G08 | tada-1 | T-DNA Insertion | T-DNA insertion is mapped to the first exon of the gene, 1597 nucleotides downstream of the initiation codon | Homozygous; Knockout | Slow growth; Pale-green; Round leaves | NA | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 19602623 |
GK-119G08 | tada-1 + ΔN-TADA | Complementation | Fuses the deaminase domain (DN-TADA) to the signal peptide of the chloroplast protein RECA1 (At1g79050) and expresses it in tada-1 under the control of the 35S promoter | NA | NA | NA | NA | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 19602623 |
Agri-140-69-2-CATMA1a58100 | RNAi (Silencing) | RNAi | NA | No TADA mRNA could be detected | NA | NA | NA | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 19602623 |
Col-0 | WT | No treatment | No treatment | No treatment | No treatment | NA | NA | Direct Sequencing of PCR Products | 50.00% | Medium | None | 19602623 |
Agri-140-69-2-CATMA1a58100 | RNAi (Suppression) | RNAi | NA | Partial TADA suppression | NA | NA | NA | Direct Sequencing of PCR Products | NA | Poor | Decreased | 19602623 |