TADA - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: TADA
Synonym: ARABIDOPSIS THALIANA TRNA ADENOSINE DEAMINASE A; ATTADA; RNA ARGININE ADENOSINE DEAMINAS; TADA1; TRNA ARGININE ADENOSINE DEAMINASE
Description: Disruption of TADA has profound effects on chloroplast translation efficiency, leading to reduced yields of chloroplast-encoded proteins and impaired photosynthetic function
Protein Family: Adenosine deaminases acting on tRNA (ADAT)
Subclass: NA
Physical Interaction: NA
Construct Structure: NA
Gene ID & Species: AT1G68720 (Arabidopsis thaliana)
Edited Gene(s): trnR
Editing Type(s): A-to-I (5)
Publication(s): [1] Arabidopsis tRNA Adenosine Deaminase Arginine Edits the Wobble Nucleotide of Chloroplast tRNAArg(ACG) and Is Essential for Efficient Chloroplast Translation, The Plant Cell, 2009. [doi:10.1105/tpc.109.066654]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G68720 Chloroplast trnR 34 Exon Arabidopsis Genome Initiative Arabidopsis Genome Initiative NA NA A-to-I ACG=>ICG T=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GK-119G08tada-1T-DNA InsertionT-DNA insertion is mapped to the first exon of the gene, 1597 nucleotides downstream of the initiation codonHomozygous; KnockoutSlow growth; Pale-green; Round leavesNANADirect Sequencing of PCR Products0.00%UneditedAbsent19602623
GK-119G08tada-1 + △N-TADAComplementationFuses the deaminase domain (DN-TADA) to the signal peptide of the chloroplast protein RECA1 (At1g79050) and expresses it in tada-1 under the control of the 35S promoterNANANANADirect Sequencing of PCR Products50.00%MediumRestored19602623
Agri-140-69-2-CATMA1a58100RNAi (Silencing)RNAiNANANANANADirect Sequencing of PCR Products0.00%UneditedAbsent19602623
Col-0WTControlControlNo mutantNormalNANADirect Sequencing of PCR Products50.00%MediumNone19602623
Agri-140-69-2-CATMA1a58100RNAi (Suppression)RNAiNANANANANADirect Sequencing of PCR ProductsNAPoorDecreased19602623
Last update: Sep 2026 (version 2.0)