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WSL5 - Plant Editosome Database - CNCB-NGDC
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Summary

Editing Factor: WSL5
Synonym: White Stripe Leaf 5
Description: The wsl5 mutation causes defects in editing of rpl2 and atpA, and splicing of rpl2 and rps12; wsl5 was impaired in chloroplast ribosome biogenesis under cold stress; WSL5 allele is required for normal chloroplast development in maintaining retrograde signaling from plastids to the nucleus under cold stress
Protein Family: PPR
Subclass: P
Physical Interaction: OsMORF8
Construct Structure: P
Gene ID & Species: Os04g0684500 (Oryza sativa)
Edited Gene(s): rpl2    atpA
Editing Type(s): C-to-U (6)
Publication(s): [1] WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress., Journal of experimental botany, 2018. [PMID=29893948]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Position Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Oryza sativa Os04g0684500 Chloroplast atpA 1148 CDS NA AB014056 NA NA C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Nanjing 11 (indica)WTControlControlNo mutantNormalSeedling10-day-oldSequencing RT-PCR products100.00%CompleteNone29893948
Nanjing 11 (indica)wsl5A point mutationA single nucleotide polymorphism (SNP; T to C) located in the conserved region caused a leucine to proline amino acid substitution in the mutant.RecessiveThe mutant develops white-striped leaves during early leaf development and is albinic when planted under cold stress.Seedling10-day-oldSequencing RT-PCR products32.84%LowDecreased29893948
Nanjing 11 (indica)WSL5-comComplementationFor complementation of the wsl5 mutation, a 2706 bp wild-type coding sequence fragment and an ~2 kb upstream sequence were amplified from variety Nanjing 11. They were cloned into the binary vector pCRecessiveTwenty-eight of 45 transgenic lines resistant to hygromycin and harboring the transgene displayed the wild-type phenotype.Seedling10-day-oldSequencing RT-PCR products100.00%CompleteRestored29893948
Oryza sativa Os04g0684500 Chloroplast rpl2 2 CDS NA AB014056 NA NA C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Nanjing 11 (indica)WTControlControlNo mutantNormalSeedling10-day-oldSequencing RT-PCR products76.19%HighNone29893948
Nanjing 11 (indica)wsl5A point mutationA single nucleotide polymorphism (SNP; T to C) located in the conserved region caused a leucine to proline amino acid substitution in the mutant.RecessiveThe mutant develops white-striped leaves during early leaf development and is albinic when planted under cold stress.Seedling10-day-oldSequencing RT-PCR products9.72%PoorDecreased29893948
Nanjing 11 (indica)WSL5-comComplementationFor complementation of the wsl5 mutation, a 2706 bp wild-type coding sequence fragment and an ~2 kb upstream sequence were amplified from variety Nanjing 11. They were cloned into the binary vector pCRecessiveTwenty-eight of 45 transgenic lines resistant to hygromycin and harboring the transgene displayed the wild-type phenotype.Seedling10-day-oldSequencing RT-PCR products75.61%HighRestored29893948
Last update: Feb 2026 (version 2.0)