WSL5 - Plant Editosome Database - BIG Data Center

WSL5 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	WSL5
Synonym:	White Stripe Leaf 5
Description:	The wsl5 mutation causes defects in editing of rpl2 and atpA, and splicing of rpl2 and rps12; wsl5 was impaired in chloroplast ribosome biogenesis under cold stress; WSL5 allele is required for normal chloroplast development in maintaining retrograde signaling from plastids to the nucleus under cold stress
Protein Family:	PPR
Subclass:	P
Construct Structure:	P
Gene ID & Species:	Os04g0684500 (Oryza sativa)
Edited Gene(s):	rpl2 atpA
Editing Type(s):	C-to-U (6)
Publication(s):	[1] WSL5, a pentatricopeptide repeat protein, is essential for chloroplast biogenesis in rice under cold stress., Journal of experimental botany, 2018. [PMID=29893948]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Oryza sativa

Os04g0684500

Chloroplast

1148

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nanjing 11 (indica)	WT	Control	Control	No mutant	Normal	Seedling	10-day-old	Sequencing RT-PCR products	100.00%	Complete	None	29893948
Nanjing 11 (indica)	wsl5	A point mutation	A single nucleotide polymorphism (SNP; T to C) located in the conserved region caused a leucine to proline amino acid substitution in the mutant.	Recessive	The mutant develops white-striped leaves during early leaf development and is albinic when planted under cold stress.	Seedling	10-day-old	Sequencing RT-PCR products	32.84%	Low	Decreased	29893948
Nanjing 11 (indica)	WSL5-com	Complementation	For complementation of the wsl5 mutation, a 2706 bp wild-type coding sequence fragment and an ~2 kb upstream sequence were amplified from variety Nanjing 11. They were cloned into the binary vector pC	Recessive	Twenty-eight of 45 transgenic lines resistant to hygromycin and harboring the transgene displayed the wild-type phenotype.	Seedling	10-day-old	Sequencing RT-PCR products	100.00%	Complete	Restored	29893948

Oryza sativa

Os04g0684500

Chloroplast

2

CDS

C-to-U

ACG=>AUG

T=>M

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nanjing 11 (indica)	WT	Control	Control	No mutant	Normal	Seedling	10-day-old	Sequencing RT-PCR products	76.19%	High	None	29893948
Nanjing 11 (indica)	wsl5	A point mutation	A single nucleotide polymorphism (SNP; T to C) located in the conserved region caused a leucine to proline amino acid substitution in the mutant.	Recessive	The mutant develops white-striped leaves during early leaf development and is albinic when planted under cold stress.	Seedling	10-day-old	Sequencing RT-PCR products	9.72%	Poor	Decreased	29893948
Nanjing 11 (indica)	WSL5-com	Complementation	For complementation of the wsl5 mutation, a 2706 bp wild-type coding sequence fragment and an ~2 kb upstream sequence were amplified from variety Nanjing 11. They were cloned into the binary vector pC	Recessive	Twenty-eight of 45 transgenic lines resistant to hygromycin and harboring the transgene displayed the wild-type phenotype.	Seedling	10-day-old	Sequencing RT-PCR products	75.61%	High	Restored	29893948

Last update: Jul 2021 (version 1.0)