WTG1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G53080

Chloroplast

ndhG

CDS

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	60.00%	High	Decreased	29140512
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	70.00%	High	Decreased	29140512
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	50 days	Bulk Sequencing	90.00%	High	Restored	29140512
SALK_006120	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	54.00%	Medium	Decreased	29140512
SALK_015164	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	50 days	Bulk Sequencing	75.00%	High	Restored	29140512
Col-0	WT	Control	Control	Control	Normal	Leaf	18 days	Bulk Sequencing	82.00%	High	None	29140512
Col-0	WT	Control	Control	Control	Normal	Leaf	50 days	Bulk Sequencing	84.00%	High	None	29140512
Col-0	WT	Control	Control	Control	Normal	Leaf	18 days	Bulk Sequencing	100.00%	Complete	None	29140512
SALK_071495	complemented transgenic line	Complementation	NA	NA	Restored a normal phenotype	Leaf	18 days	Bulk Sequencing	100.00%	Complete	Restored	29140512

Arabidopsis thaliana

AT5G53080

Chloroplast

petL

CDS

C-to-U

CCU=>CUU

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	40.00%	Medium	Decreased	29140512
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	45.00%	Medium	Decreased	29140512
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	50 days	Bulk Sequencing	80.00%	High	Restored	29140512
SALK_006120	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	67.00%	High	Decreased	29140512
SALK_015164	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	50 days	Bulk Sequencing	88.00%	High	Restored	29140512
Col-0	WT	Control	Control	Control	Normal	Leaf	18 days	Bulk Sequencing	90.00%	High	None	29140512
Col-0	WT	Control	Control	Control	Normal	Leaf	50 days	Bulk Sequencing	91.00%	High	None	29140512
Col-0	WT	Control	Control	Control	Normal	Leaf	18 days	Bulk Sequencing	100.00%	Complete	None	29140512
SALK_071495	complemented transgenic line	Complementation	NA	NA	Restored a normal phenotype	Leaf	18 days	Bulk Sequencing	100.00%	Complete	Restored	29140512

Editing Factor:	WTG1
Synonym:	WHITE TO GREEN 1
Description:	Encoding a chloroplast localized TPR protein required for chloroplast biogenesis. Mutants are delayed in greening and defective in splicing petL and ndhG; WTG1 does not itself bind RNA but it does bind known editing factors MORF8 and MORF9
Protein Family:	TPR
Subclass:	NA
Construct Structure:	NA
Gene ID & Species:	AT5G53080 (Arabidopsis thaliana)
Edited Gene(s):	petL ndhG
Editing Type(s):	C-to-U (18)
Publication(s):	[1] A novel tetratricopeptide repeat protein, WHITE TO GREEN1, is required for early chloroplast development and affects RNA editing in chloroplasts., Journal of experimental botany, 2017. [PMID=29140512]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details