WTG1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: WTG1
Synonym: WHITE TO GREEN 1
Description: Encodes a chloroplast localized TPR protein required for chloroplast biogenesis. Mutants are delayed in greening and defective in splicing petL and ndhG. WTG1 does not itself bind RNA but it does bind known editing factors MORF8 and MORF9.
Protein Family: TPR
Subclass: NA
Construct Structure: NA
Gene ID & Species: AT5G53080 (Arabidopsis thaliana)
Edited Gene(s): petL    ndhG
Editing Type(s): C-to-U (18)
Publication(s): [1] A novel tetratricopeptide repeat protein, WHITE TO GREEN1, is required for early chloroplast development and affects RNA editing in chloroplasts, Journal of Experimental Botany, 2017. [PMID=29140512]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G53080 Chloroplast ndhG 50 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_071495wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf18 daysBulk Sequencing60.00%HighDecreased29140512
SALK_071495wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf18 daysBulk Sequencing70.00%HighDecreased29140512
SALK_071495wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf50 daysBulk Sequencing90.00%HighRestored29140512
SALK_006120wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf18 daysBulk Sequencing54.00%MediumDecreased29140512
SALK_015164wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf50 daysBulk Sequencing75.00%HighRestored29140512
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf18 daysBulk Sequencing82.00%HighNone29140512
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf50 daysBulk Sequencing84.00%HighNone29140512
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf18 daysBulk Sequencing100.00%CompleteNone29140512
SALK_071495complemented transgenic lineComplementationNANARestored a normal phenotypeLeaf18 daysBulk Sequencing100.00%CompleteRestored29140512
Arabidopsis thaliana AT5G53080 Chloroplast petL 5 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_071495wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf18 daysBulk Sequencing40.00%MediumDecreased29140512
SALK_071495wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf18 daysBulk Sequencing45.00%MediumDecreased29140512
SALK_071495wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf50 daysBulk Sequencing80.00%HighRestored29140512
SALK_006120wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf18 daysBulk Sequencing67.00%HighDecreased29140512
SALK_015164wtg1T-DNA InsertionT-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codonKnockout; HomozygousDwarf and albino seedlings with serrated leavesLeaf50 daysBulk Sequencing88.00%HighRestored29140512
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf18 daysBulk Sequencing90.00%HighNone29140512
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf50 daysBulk Sequencing91.00%HighNone29140512
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf18 daysBulk Sequencing100.00%CompleteNone29140512
SALK_071495complemented transgenic lineComplementationNANARestored a normal phenotypeLeaf18 daysBulk Sequencing100.00%CompleteRestored29140512
Last update: Jul 2021 (version 1.0)