WTG1 - Plant Editosome Database

Summary

Editing Factor:	WTG1
Synonym:	WHITE TO GREEN 1
Description:	Disruption of WTG1 suppresses plant growth, retards leaf greening and chloroplast development, and represses photosynthetic gene expression, but complemented expression of WTG1 restored a normal phenotype; Moreover, WTG1 protein is associated with the organelle RNA editing factors MORF8 and MORF9, and RNA editing of the plastid petL-5 and ndhG-50 transcripts was affected in wtg1 mutants
Protein Family:	Tetratricopeptide repeat (TPR) protein
Subclass:	NA
Physical Interaction:	MORF8; MORF9
Construct Structure:	NA
Gene ID & Species:	AT5G53080 (Arabidopsis thaliana)
Edited Gene(s):	petL ndhG
Editing Type(s):	C-to-U (18)
Publication(s):	[1] A novel tetratricopeptide repeat protein, WHITE TO GREEN1, is required for early chloroplast development and affects RNA editing in chloroplasts, Journal of Experimental Botany, 2017. [doi:10.1093/jxb/erx383]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT5G53080

Chloroplast

ndhG

CDS

Arabidopsis Genome Initiative

AP000423

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	60.00%	High	Decreased	29140512
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	70.00%	High	Decreased	29140512
SALK_071495	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	50 days	Bulk Sequencing	90.00%	High	Restored	29140512
SALK_006120	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	18 days	Bulk Sequencing	54.00%	Medium	Decreased	29140512
SALK_015164	wtg1	T-DNA Insertion	T-DNA is inserted at the end of the third exon, 804 bp downstream from the initiation codon	Knockout; Homozygous	Dwarf and albino seedlings with serrated leaves	Leaf	50 days	Bulk Sequencing	75.00%	High	Restored	29140512
Col-0	WT	Control	Control	No mutant	Normal	Leaf	18 days	Bulk Sequencing	82.00%	High	None	29140512
Col-0	WT	Control	Control	No mutant	Normal	Leaf	50 days	Bulk Sequencing	84.00%	High	None	29140512
Col-0	WT	Control	Control	No mutant	Normal	Leaf	18 days	Bulk Sequencing	100.00%	Complete	None	29140512
SALK_071495	complemented transgenic line	Complementation	NA	NA	Restored a normal phenotype	Leaf	18 days	Bulk Sequencing	100.00%	Complete	Restored	29140512