qKW9 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

Zm00001d048451

Chloroplast

Intron

B73_RefGen_v4

Genomic position

65407

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	Before pollination	RNA-Seq	0.00%	Unedited	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	22 DAP	RNA-Seq	0.00%	Unedited	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	30 DAP	RNA-Seq	0.00%	Unedited	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	Before pollination	RNA-Seq	72.73%	High	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	22 DAP	RNA-Seq	88.89%	High	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	30 DAP	RNA-Seq	100.00%	Complete	None	32482908

Zea mays

Zm00001d048451

Chloroplast

ndhB

CDS

B73_RefGen_v4

Genomic position

90736
132001

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	Before pollination	RNA-Seq	0.00%	Unedited	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	22 DAP	RNA-Seq	0.00%	Unedited	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	30 DAP	RNA-Seq	0.00%	Unedited	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	Before pollination	RNA-Seq	100.00%	Complete	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	22 DAP	RNA-Seq	100.00%	Complete	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	30 DAP	RNA-Seq	100.00%	Complete	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	Before pollination	RNA-Seq	0.00%	Unedited	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	22 DAP	RNA-Seq	0.00%	Unedited	None	32482908
Small Kernel (SK)	NIL-SK	Control	Control	No mutant	The NIL-SK kernels weighed only ~3 g less per hundred kernels than NIL-ZHENG58, and their kernel morphology, starch granule structure, and plant morphology were similar; 2-week-old seedlings of NIL-SK were smaller than NIL-ZHENG58, possibly as a result of smaller NIL-SK kernels that provided less nutrition to support their heterotrophic growth, which relies on seed-derived nutrients; At the mature stage, NIL-SK and NIL-ZHENG58 plants had similar plant architecture; NIL-SK plants had the same kernel row number but fewer kernels per row compared to NIL-ZHENG58 plants, resulting in smaller ears with fewer kernels	Leaf	30 DAP	RNA-Seq	0.00%	Unedited	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	Before pollination	RNA-Seq	97.05%	High	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	22 DAP	RNA-Seq	98.79%	High	None	32482908
Zheng58	NIL-ZHENG58	Control	Control	No mutant	Normal	Leaf	30 DAP	RNA-Seq	98.60%	High	None	32482908

Editing Factor:	qKW9
Synonym:	NA
Description:	In a null qkw9 background, C-to-U editing of ndhB was abolished, and photosynthesis was reduced, resulting in less maternal photosynthate available for grain filling
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-DYW
Gene ID & Species:	Zm00001d048451 (Zea mays)
Edited Gene(s):	ndhB NA
Editing Type(s):	C-to-U (18)
Publication(s):	[1] The Kernel Size-Related Quantitative Trait Locus Encodes a Pentatricopeptide Repeat Protein That Aaffects Photosynthesis and Grain Filling., Plant physiology, 2020. [PMID=32482908]

Summary

Editing Details