The dataset included a total of 1991 cells, the parameters adopted were as follows: resolution=1, PCs=1:20, MT<10%.

Receptor genes and their paired ligand genes were collected from CelltalkDB, SingleCellSingalR, Cellinker, Cell-Cell Interaction Database and Armingol E, et al., 2021. The following figures only demonstrated the expression of receptor genes and ligand genes supported by three or more databases or other sources, and the corresponding relationship between ligands and receptors were shown in the following table.

Ligand	Receptor	Ligand	Receptor	Ligand	Receptor	Ligand	Receptor
ADAM10	CD44, IL6R, TREM2	ADAM12	ITGB1	ADAM15	ITGB1	ADAM17	ERBB4, IL6R, ITGB1
ADAM2	ITGB1, ITGB7	ADAM9	ITGB1	AGRN	ITGB1	AMH	ACVR1, AMHR2, BMPR1A, EGFR
ANG	EGFR	ANGPT1	ITGB1	ANXA1	ADRA2A, DYSF, EGFR, FPR1, FPR2, FPR3, GRM7, LXA4, MTNR1A	APOC3	TLR2
APOE	LRP5, TREM2	APP	CD74, FPR2	AREGB	EGFR	AREG	EGFR
ARF4	EGFR	ARTN	GFRA1, GFRA2, GFRA3, RET	B2M	KLRC1, KLRC2, KLRD1	BCAN	EGFR
BGN	TLR2	BMP10	BMPR1B, BMPR2	BMP15	BMPR1B, BMPR2	BMP1	BMPR1B, BMPR2
BMP2	ACVR1, BMPR1B, BMPR2	BMP3	BMPR1B, BMPR2	BMP4	BMPR1B, BMPR2	BMP5	BMPR1B, BMPR2
BMP6	ACVR1, BMPR1B, BMPR2	BMP7	ACVR1, BMPR1B, BMPR2	BMP8A	BMPR1B, BMPR2	BMP8B	BMPR1A, BMPR1B, BMPR2, PLAUR
BTC	EGFR, ERBB4	C4BPA	BMPR2	CALM1	EGFR	CALM2	EGFR
CALM3	EGFR	CAMP	EGFR, FPR2	CCN1	TLR2	CCN2	EGFR, ERBB4
CCN3	PLXNA1	CD14	ITGB1	CDH1	CDH2, EGFR, ERBB3, IGF1R, ITGAE, ITGB7, KLRG1, LRP5, PTPRF, PTPRM, TRPV4	CEACAM1	EGFR
CEACAM6	EGFR	CEL	CXCR4	CHAD	ITGB1	CLCF1	IL6ST
CMTM8	EGFR	CNTF	EGFR, IL6R, IL6ST	CNTN2	CNTN1, CNTN2, CNTNAP2, NRCAM, NRP1	COL11A1	ITGB1
COL14A1	CD44	COL16A1	ITGB1	COL18A1	ITGB1, KDR	COL1A1	CD44, ITGA1, ITGB1
COL1A2	CD44, ITGA1, ITGB1	COL2A1	ITGA1, ITGB1	COL3A1	ITGB1	COL4A1	ITGA1, ITGB1
COL4A3	ITGA1, ITGB1	COL4A4	ITGA1, ITGB1	COL4A5	ITGA1, ITGB1	COL4A6	ITGA1, ITGB1
COL5A1	ITGA1, ITGB1	COL5A2	ITGA1, ITGB1	COL6A1	ITGA1, ITGB1	COL6A2	ITGA1, ITGB1
COL6A3	ITGA1, ITGB1	COL7A1	ITGB1	COL8A1	ITGA1	COL9A1	ITGB1
COL9A2	ITGB1	COL9A3	ITGB1	COMP	ITGB1	CSF2	CSF1R, CSF2RA, CSF2RB, CSF3R, IL3RA, ITGA9, ITGB1, SDC2
CTF1	IL6ST	CXCL12	CXCR4, ITGB1	CXCL14	CXCR4	CXCL1	CXCR2
CXCL2	CXCR2	CXCL3	CXCR2	CXCL5	CXCR2	CXCL6	CXCR2
CXCL7	CXCR2	CXCL8	CXCR2, KDR	DCN	EGFR, ERBB4	DKK1	LRP5
DKK4	LRP5	DSPP	ITGB1	EBI3	IL6ST	EFEMP1	EGFR
EGF	ADRB2, AXL, CAV1, EGFR, ERBB2, ERBB3, ERBB4, FSHR, LDLR, LRP2, NRP1, PLD2, RHBDL2	EPGN	EGFR	EREG	EGFR, ERBB4	F13A1	ITGB1
FARP2	PLXNA1	FBLN1	ITGB1	FBN1	ITGB1	FGA	ITGB1
FGB	ITGB1	FGF13	EGFR	FGF1	CD44, EGFR, NRP1	FGF2	CD44, NRP1
FGF4	NRP1	FGF7	NRP1	FGG	ITGB1	FGL1	EGFR
FN1	CD44, ITGB1, ITGB7	FST	BMPR1B, BMPR2	GDF11	BMPR1B, BMPR2	GDF15	RET
GDF2	ACVR1, BMPR2	GDF5	ACVR1, BMPR1B, BMPR2	GDF6	BMPR1B, BMPR2	GDF7	BMPR2
GDF9	ACVR1B, ACVR2A, BMPR1A, BMPR1B, BMPR2, FXYD6, ORAI2, TGFBR1	GDNF	GFRA2, GFRA3, RET	GNAI2	CXCR2, EGFR	GREM1	KDR
GRN	EGFR	GSTP1	EGFR	H60a	KLRK1	HAS2	CD44
HBEGF	CD44, EGFR, ERBB4	HGF	CD44, ITGB1, NRP1	HLA-B	KLRD1	HLA-E	KIR3DL1, KLRC1, KLRC2, KLRC3, KLRD1, KLRK1
HLAE	KLRK1	HLA-G	KLRC1, KLRD1	HMGB1	CXCR4, TLR2	HRAS	TLR2
HSP90AA1	EGFR	HSP90B1	TLR2	HSPG2	ITGB1	ICAM1	EGFR, IL2RA, IL2RG
ICAM4	ITGB1	IFLNL2	IL10RB	IFNG	IFNGR1, IFNGR2	IFNL1	IL10RB
IFNL2	IL10RB	IFNL3	IL10RB	IGKV1-16	CNTN2	IL10	IL10RA, IL10RB, SIRPG
IL11	IL11RA, IL11R complex, IL6ST	IL13	IL2RG, IL4R	IL15	IL15RA, IL2RA, IL2RB, IL2RG	IL17B	IL17RB
IL17C	IL17RA, IL17RE	IL19	IL20RA	IL20	IL20RA, IL22RA1	IL21	IL21R, IL2RG
IL22	IL10RA, IL10RB, IL21R, IL22RA1, IL22RA2	IL24	IL10RB, IL20RA, IL22RA1	IL25	IL17RA, IL17RB, MED30, NCOR1	IL26	IL10RB, IL20RA
IL27 complex	IL6ST	IL28B	IL10RB	IL2	CD53, GYPE, IL2RA, IL2RB, IL2RG, NGFR	IL31	IL31RA, OSMR
IL33	IL1RAP, IL1RL1	IL3	CSF2RB, IL3RA	IL4	CD53, IL13RA1, IL13RA2, IL2RG, IL4R	IL5	CSF2RB, IL5RA
IL6	F3, HRH1, IL6R, IL6ST	IL7	IL2RG, IL7R	IL9	IL2RG, IL9R	INHA	ACVR1
INHBA	ACVR1	INHBB	ACVR1	INHBC	ACVR1	JAM3	ITGB1
L1CAM	EGFR	LAMA1	ITGA1, ITGB1	LAMA2	ITGA1, ITGB1	LAMA3	ITGB1
LAMA4	ITGB1	LAMA5	ITGB1	LAMB1	ITGA1, ITGB1	LAMB2	ITGB1
LAMB3	ITGB1	LAMC1	ITGA1, ITGB1	LAMC2	ITGB1	LAMC3	ITGB1
LGALS1	ITGB1	LGALS3BP	ITGB1	LGALS9	CD44	LIF	IL6ST
LPL	CD44	LRIG1	EGFR	LRIG2	EGFR	LUM	ITGB1
MADCAM1	CD44, ITGB7	MATN1	ITGA1	MDK	ITGB1	MGI:109477	KLRK1
MGI:95957	KLRC1, KLRC2, KLRD1	MICA	KLRK1	MICB	KLRK1	MIF	CD44, CD74, CXCR2, CXCR4, EGFR
MMP1	CD44	MMP7	CD44, ERBB4	MMP9	CD44	NID1	ITGB1
Npnt complex 1	ITGB1	Npnt complex 2	ITGB1	NPNT	ITGB1	NRG1	EGFR, ERBB4
NRG2	ERBB4	NRG3	ERBB4	NRG4	EGFR, ERBB4	NRTN	GFRA1, GFRA2, GFRA3, RET
OSM	IL6ST, LIFR, OSMR	PDGFC	FLT1, KDR	PGF	FLT1, NRP1, NRP2	PIGF	FLT1
PKM	CD44	PLAU	ITGB1	PLG	FLT1, ITGB1	PPBP	CXCR2
PRG4	CD44	PSEN1	CD44	PSPN	GFRA1, GFRA2, GFRA3, GFRA4, RET, SDC3	RAET1E	KLRK1
RAET1G	KLRK1	RAET1L	KLRK1	RELN	ITGB1	RGMA	BMPR1B, BMPR2
RGMB	BMPR1B, BMPR2	RNASE2	TLR2	S100A4	EGFR	SAA1	FPR2, TLR2
SDF1	CXCR4	SEMA3A	NRP1, NRP2, PLXNA1	SEMA3B	NRP1, NRP2	SEMA3C	NRP1, NRP2, PLXND1
SEMA3D	NRP1	SEMA3E	NRP1	SEMA3F	NRP1, NRP2, PLXNA1	SEMA3G	NRP2
SEMA4A	NRP1	SEMA4F	NRP2	SEMA6D	KDR, PLXNA1, TREM2, TYROBP	SEMA7A	ITGA1, ITGB1, PLXNC1
SFTPA1	TLR2	SORBS1	ITGA1	SOST	LRP5	SPINK1	EGFR
SPP1	CD44, ITGB1	ST6GAL1	EGFR	TGFA	EGFR, ERBB4	TGFB1	CXCR4, EGFR, ITGB1
TGFB2	ACVR1	TGFB3	ITGB1	TGM2	ITGB1	THBS1	ITGB1, LRP5
THBS2	ITGB1	TIMP2	CD44, ITGB1	TIMP3	CD44, KDR	TNC	EGFR, ITGB1
TRBV20OR9-2	CXCR4	TSLP	CRLF2, IL7R	UCN2	IL10RB	UCN3	IL10RB
ULBP1	HCST, KLRK1	ULBP2	KLRK1	ULBP3	KLRK1	VASP	CXCR2
VCAM1	ITGB1, ITGB7	VCAN	CD44, EGFR, ITGB1, TLR2	VEGFA	EGFR, EPHB2, FLT1, GPC1, GRIN2B, ITGA9, ITGAV, ITGB1, ITGB3, KDR, NRP1, NRP2, RET, SIRPA, TYRO3	VEGFB	FLT1, NRP1, RET
VEGFC	FLT1, ITGB1, KDR, NRP2	VEGFD	FLT1, ITGA1, ITGB1, KDR, NRP2	VIM	CD44	VTN	ITGB1, KDR
WNT1	LRP5	WNT3A	LRP5	WNT5A	LRP5	WNT6	FZD4, FZD7, FZD8, FZD9, LRP5
WNT7A	FZD1, FZD10, FZD2, FZD3, FZD5, FZD9, LDLR, LRP5, LRP6, RECK	WNT7B	FZD10, LRP5	WNT8A	LRP5	WNT8B	LRP5
ZG16B	CXCR4, TLR2, TLR4, TLR5	ZP3	EGFR	-	-	-	-

Oncogenes and tumor suppressor genes (TSGs) were collected from Cancer Gene Census (CGC), OncoKB, Network of Cancer Genes (NCG), TSGene, IntOGene and Volkan Okur and Wendy K. Chung, 2017. The following figures only displayed the expression of oncogenes and TSGs supported by three or more databases or other sources.