HemAtlas
Mapping developmental
hematopoiesis across stages, species and models
HemSC00000066
Dataset Information
Omics:
Single cell RNA-seq
Omics Detail:
10x Genomics
Species:
Zebrafish
Organ:
KM (kidney marrow)
Cell Number:
34492
Gene Number:
27777
Genome Reference:
GRCz11
Data Normalization:
SCT
Sequencing Platform:
10x Genomics
Cell Sorting Strategies:
In the case of the marrows, live cell sorts were divided into two fractions based on forward scatter and side scatter characteristics—a lymphoid and progenitor fraction and a granulocyte fraction. This allowed for the relative enrichment of lymphoid and progenitor cells in the overall analysis, advantageous for our goal to investigate B cell developmental trajectories. By nature of this sorting strategy, our marrow data does not reflect whole kidney marrow composition. Between 50,000 and 100,000 cells were sorted per sample.
Publication
Single-cell analyses reveal early thymic progenitors and pre-B cells in zebrafish.
Rubin SA, Baron CS, Pessoa Rodrigues C, Duran M, Corbin AF, Yang SP, Trapnell C, Zon LI.
Volume:219 Issue:9 The Journal of experimental medicine
| 2022-08-08 | [35938989]