HemAtlas
Mapping developmental
hematopoiesis across stages, species and models
HemSC00000068
Dataset Information
Omics:
Single cell RNA-seq
Omics Detail:
10x Genomics
Species:
Mouse
Organ:
Bone marrow
Cell Number:
4897
Gene Number:
17479
Genome Reference:
mm10plus
Data Normalization:
SCT
Sequencing Platform:
10x Genomics
Cell Sorting Strategies:
whole BM cells (depleted of RBCs through Ficollseparation), For a higher resolution view of the various populations in the BM, they subfractionated the BM using FACS into five different populations: (l) LinKit*Scal* HPCs that are known to include rare long-term HSCs and much more abundant short-term multipotent progenitors (MPPs), (2) Terl19-B220* cells that include both immature and mature B cells, (3)Ter1 19-Cd90*Cd2* cells that include immature T cells and NK cells, (4) Ter119 Mac1*Grl"i cellsthat include predominantly granulocytes and (5)TER119-Macl*GrMidlo cells enriched inmonocytes.
Publication
Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris.
Tabula Muris Consortium, Overall coordination, Logistical coordination, Organ collection and processing, Library preparation and sequencing, Computational data analysis, Cell type annotation, Writing group, Supplemental text writing group, Principal investigators.
Volume:562 Issue:7727 Nature
| 2018-10-03 | [30283141]