Basic information
Locus name	AT1G69270
Alias	RPK1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:protein kinase and phosphatases
Effect for Senescence	promote
Gene Description	leucine-rich repeat family protein / protein kinase family protein contains Pfam domains PF00560: Leucine Rich Repeat and PF00069: Protein kinase domain
Evidence	Genomic evidence:microarray data [Ref 1]; Genetic evidence: mutant and transgene [Ref 2]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85 2: Lee IC, Hong SW, Whang SS, Lim PO, Nam HG, Koo JC Age-dependent action of an ABA-inducible receptor kinase, RPK1, as a positive regulator of senescence in Arabidopsis leaves. Plant Cell Physiol. 2011 Apr;52(4):651-62
Gene Ontology	biological process protein phosphorylation molecular function ATP binding protein kinase activity protein serine/threonine kinase activity
Sequence	AT1G69270.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name rpk1-3 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type Ds insertion;T-DNA
Mutated 2 Mutant name rpk1-4 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type Ds insertion;T-DNA
Mutated 3 Mutant name iRPK1 Mutant/Transgenic transgenic Ecotype Col-0 Mutagenesis type Transgene
Mutated 4 Mutant name iRPK1-GFP Mutant/Transgenic transgenic Ecotype Col-0 Mutagenesis type Transgene
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G69270.1  miRNA: ath-miR864-3p miRNA: ath-miR864-3p mfe: -23.4 kcal/mol  p-value: 0.002193  position:  126  target 5' U        U           C 3'             UUUAGGGU UUGUUGAUUUU              AAGUUCCA AAUAACUGAAA   miRNA  3' G        U           U 5'
Cross Link
Database Entry ID E-value Start End InterPro ID Description Pfam PF08263 3.1E-14 31 70 IPR013210 Leucine-rich repeat-containing N-terminal, type 2 SUPERFAMILY SSF52058 6.3E-17 31 159 No hit NA PANTHER PTHR24420 0.0 105 538 No hit NA SUPERFAMILY SSF56112 2.0E-74 243 532 IPR011009 Protein kinase-like domain ProSiteProfiles PS50011 39.6 261 535 IPR000719 Protein kinase, catalytic domain SMART SM00220 1.6E-37 261 535 IPR002290 Serine/threonine- / dual specificity protein kinase, catalytic domain Pfam PF00069 4.3E-49 264 528 IPR000719 Protein kinase, catalytic domain ProSitePatterns PS00107 NA 267 289 IPR017441 Protein kinase, ATP binding site ProSitePatterns PS00108 NA 381 393 IPR008271 Serine/threonine-protein kinase, active site
Mutant Image
Reactive oxygen species (ROS);A mutant version of RPK1-GFP (mRPK1-GFP), having a partial deletion in the kinase domain, was not able to lead to the activation of the SIRK promoter
Subcellular Localization
Localization	plasma membrane
Evidence	SUBAcon
Pubmed ID	23180787