Basic information
GenBank ID	AB040454
Alias	AsNODF32
Organism	Chinese Milk Vetch (Astragalus sinicus)
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:cysteine proteinase
Effect for Senescence	promote
Gene Description	Asnodf32, encoding a nodule-specific cysteine proteinase in Astragalus sinicus, is probably involved in nodule senescence.silencing of Asnodf32 delayed root nodule and bacteroid senescence.
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Li Y, Zhou L, Li Y, Chen D, Tan X, Lei L, Zhou J A nodule-specific plant cysteine proteinase, AsNODF32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume Astragalus sinicus. New Phytol. 2008;180(1):185-92 2: Naito Y, Fujie M, Usami S, Murooka Y, Yamada T The involvement of a cysteine proteinase in the nodule development in Chinese milk vetch infected with Mesorhizobium huakuii subsp. rengei. Plant Physiol. 2000 Nov;124(3):1087-96
Gene Ontology	biological process proteolysis molecular function cysteine-type peptidase activity
Sequence	AB040454.1 | mRNA | CDS | Protein
Mutant information
Mutant name	Asnodf32-silenced line
Mutant/Transgenic	transgenic
Ecotype	Astragalus sinicus L.
Mutagenesis type	RNAi
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR12411 1.8E-171 7 342 IPR013128 Peptidase C1A, papain SUPERFAMILY SSF54001 9.8E-113 33 342 No hit NA SMART SM00848 1.4E-20 39 96 IPR013201 Proteinase inhibitor I29, cathepsin propeptide Pfam PF08246 8.6E-16 40 96 IPR013201 Proteinase inhibitor I29, cathepsin propeptide Pfam PF00112 3.6E-86 125 342 IPR000668 Peptidase C1A, papain C-terminal SMART SM00645 7.5E-129 125 342 IPR000668 Peptidase C1A, papain C-terminal PRINTS PR00705 1.4E-9 143 158 IPR000668 Peptidase C1A, papain C-terminal ProSitePatterns PS00139 NA 143 154 IPR000169 Cysteine peptidase, cysteine active site ProSitePatterns PS00639 NA 284 294 IPR025660 Cysteine peptidase, histidine active site PRINTS PR00705 1.4E-9 286 296 IPR000668 Peptidase C1A, papain C-terminal PRINTS PR00705 1.4E-9 302 308 IPR000668 Peptidase C1A, papain C-terminal ProSitePatterns PS00640 NA 302 321 IPR025661 Cysteine peptidase, asparagine active site