Basic information
Locus name	AT1G21400
Alias	E1A1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling
Effect for Senescence	delay
Gene Description	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein.oxidation-reduction process
Evidence	Genetic evidence:Mutant [Ref 1]
References	1: Ishizaki K, Larson TR, Schauer N, Fernie AR, Graham IA, Leaver CJ. The critical role of Arabidopsis electron-transfer flavoprotein:ubiquinone oxidoreductase during dark-induced starvation. Plant Cell. 2005 Sep;17(9):2587-600. Epub 2005 Jul 29.
Gene Ontology	biological process metabolic process molecular function oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
Sequence	AT1G21400.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	e1a1-1 (SALK_071680C)
Mutant/Transgenic	mutant
Ecotype	Col-0
Mutagenesis type	T-DNA
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G21400.1  miRNA: ath-miR472 miRNA: ath-miR472 mfe: -27.4 kcal/mol  p-value: 0.010647  position:  1263  target 5' C               A      U 3'             GG AU GGUGGAGUG GGAAGA              CC UA CCGCCUCAU CCUUUU   miRNA  3'    A  C                U 5'
Ortholog Group
Ortholog Groups: OG5_128261	Accession Taxon NP_173562 ( AT1G21400 ) Arabidopsis thaliana NP_568209 Arabidopsis thaliana NP_974756 Arabidopsis thaliana CMH008C Cyanidioschyzon merolae strain 10D 102917 Chlamydomonas reinhardtii NP_001066324 Oryza sativa Japonica Group estExt_Genewise1.C_2070062 Physcomitrella patens subsp. patens 29648.m001932 Ricinus communis 29992.m001413 Ricinus communis fgenesh1_pm.C_chr_17000012 Thalassiosira pseudonana CCMP1335 XP_002955721 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11516 1.1E-234 40 467 No hit NA SUPERFAMILY SSF52518 4.8E-107 103 467 No hit NA Pfam PF00676 2.9E-96 135 430 IPR001017 Dehydrogenase, E1 component Coils Coil NA 411 432 No hit NA
Mutant Image
High levels of free branched-chain amino acids in leaves subjected to prolonged darkness, early senescence in prolonged darkness
Subcellular Localization
Localization	mitochondrion
Evidence	SUBAcon
Pubmed ID	23180787