Basic information
Locus name	At4g04955
Alias	AtALN
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling
Effect for Senescence	delay
Gene Description	Encodes an allantoinase which is involved in allantoin degradation and assimilation.
Evidence	Genetic evidence [Ref 1]
References	1: Soltabayeva A, Srivastava S, Kurmanbayeva A, Bekturova A, Fluhr R, Sagi M. Early Senescence in Older Leaves of Low Nitrate-Grown Atxdh1 Uncovers a Role for Purine Catabolism in N Supply. Plant Physiol. 2018 Nov;178(3):1027-1044. doi: 10.1104/pp.18.00795. Epub 2018 Sep 6.
Gene Ontology	biological process allantoin catabolic process molecular function allantoinase activity cobalt ion binding hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds hydrolase activity zinc ion binding
Pathway	KEGG map00230 (EC 3.5.2.5) MetaCyc PWY-5697
Sequence	AT4G04955.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	Ataln
Mutant/Transgenic	mutant
Ecotype	Col-0
Mutagenesis type	T-DNA
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_127802	Accession Taxon NP_567276 ( At4g04955 ) Arabidopsis thaliana 190406 Chlamydomonas reinhardtii ACO61596 Micromonas sp. RCC299 NP_001054286 Oryza sativa Japonica Group 1700010243 Ostreococcus tauri e_gw1.7.82.1 Physcomitrella patens subsp. patens estExt_Genewise1.C_1050065 Physcomitrella patens subsp. patens estExt_fgenesh1_pg.C_360198 Physcomitrella patens subsp. patens 28964.m000072 Ricinus communis 29851.m002399 Ricinus communis 34923.m000020 Ricinus communis XP_002951580 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description SUPERFAMILY SSF51338 1.3E-22 7 100 IPR011059 Metal-dependent hydrolase, composite domain PANTHER PTHR11647 7.8E-229 28 493 No hit NA TIGRFAM TIGR03178 5.7E-144 47 501 IPR017593 Allantoinase Pfam PF01979 3.7E-21 96 445 IPR006680 Amidohydrolase 1 SUPERFAMILY SSF51556 1.3E-89 100 431 No hit NA SUPERFAMILY SSF51338 1.3E-22 431 485 IPR011059 Metal-dependent hydrolase, composite domain
Mutant Image
Older leaves of the Ataln mutant exhibited early senescence, lower soluble protein and lower organic-N levels as compared to wild-type (WT) older leaves when grown with 1 mM nitrate but were comparable to WT under 5 mM nitrate.
Subcellular Localization
Localization	extracellular
Evidence	SUBAcon
Pubmed ID	23180787