Leaf Senescence DataBase

Basic information

Locus name

AT1G10070

Alias

ATBCAT-2

Organism

Arabidopsis thaliana

Taxonomic identifier

[NCBI]

Function category

Nutrient recycling

Effect for Senescence

unclear

Gene Description

branched-chain amino acid aminotransferase 2 / branched-chain amino acid transaminase 2 (BCAT2) identical to SP|Q9M439 Branched-chain amino acid aminotransferase 2, chloroplast precursor (EC 2.6.1.42) (Atbcat-2) {Arabidopsis thaliana}; contains Pfam profile: PF01063 aminotransferase class IV

Evidence

Genomic evidence:microarray data [Ref 1]

References

1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ
Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis.
Plant J. 2005 May;42(4):567-85

2: Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston
High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation.
Plant Cell 2011 Mar;23(3):873-94

Gene Ontology

biological process	branched-chain amino acid metabolic process metabolic process
molecular function	branched-chain-amino-acid transaminase activity catalytic activity

Pathway

Reactome	REACT_13
KEGG	map00280 (EC 2.6.1.42) map00290 (EC 2.6.1.42) map00770 (EC 2.6.1.42) map00966 (EC 2.6.1.42)
MetaCyc	PWY-5057 PWY-5076 PWY-5078

Sequence

Microarray

Sampling

Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ.
Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis.
Plant J. 2005 May;42(4)

Comparation

Legends:
Col: wild type, indicates the ratio of expression in senescing leaves/green leaves.
NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type.
D/L: ratio of DARK 5d/control.
CD: Cell Death, ratio of starved cell suspension culture/control.
Genes showing at least 3 fold (ratio) up regulation during leaf senescence.

miRNA Interaction

Details

target: AT1G10070.1
miRNA: ath-miR843
miRNA: ath-miR843
mfe: -31.0 kcal/mol
p-value: 0.003787

position:  562
target 5' A   G             G     C 3'
           UCC GAUGAAGCUUGGC CUGAA
           AGG UUACUUCGAGCUG GAUUU
miRNA  3'                           5'

target: AT1G10070.2
miRNA: ath-miR843
miRNA: ath-miR843
mfe: -31.0 kcal/mol
p-value: 0.003568

position:  478
target 5' A   G             G     C 3'
           UCC GAUGAAGCUUGGC CUGAA
           AGG UUACUUCGAGCUG GAUUU
miRNA  3'                           5'

target: AT1G10070.3
miRNA: ath-miR156h
miRNA: ath-miR156h
mfe: -24.9 kcal/mol
p-value: 0.007770

position:  230
target 5' A      G           C 3'
           UGUUCU UUUUUUUUGUC
           ACGAGA AAAGAAGACAG
miRNA  3' C      G           U 5'

target: AT1G10070.3
miRNA: ath-miR843
miRNA: ath-miR843
mfe: -31.0 kcal/mol
p-value: 0.003854

position:  588
target 5' A   G             G     C 3'
           UCC GAUGAAGCUUGGC CUGAA
           AGG UUACUUCGAGCUG GAUUU
miRNA  3'                           5'

Ortholog Group

Ortholog Groups: OG5_126731

Accession	Taxon
NP_001031015 ( AT1G10070 )	Arabidopsis thaliana
NP_001031016	Arabidopsis thaliana
NP_001118802	Arabidopsis thaliana
NP_172478	Arabidopsis thaliana
NP_187170	Arabidopsis thaliana
NP_201379	Arabidopsis thaliana
NP_566923	Arabidopsis thaliana
NP_568496	Arabidopsis thaliana
CMN181C	Cyanidioschyzon merolae strain 10D
CMO144C	Cyanidioschyzon merolae strain 10D
187392	Chlamydomonas reinhardtii
196279	Chlamydomonas reinhardtii
206169	Chlamydomonas reinhardtii
ACO66090	Micromonas sp. RCC299
ACO70611	Micromonas sp. RCC299
NP_001048685	Oryza sativa Japonica Group
NP_001049461	Oryza sativa Japonica Group
NP_001050150	Oryza sativa Japonica Group
NP_001053540	Oryza sativa Japonica Group
NP_001056295	Oryza sativa Japonica Group
0800010219	Ostreococcus tauri
e_gw1.04.00.261.1	Ostreococcus tauri
e_gw1.163.56.1	Physcomitrella patens subsp. patens
e_gw1.180.30.1	Physcomitrella patens subsp. patens
e_gw1.2139.1.1	Physcomitrella patens subsp. patens
e_gw1.34.119.1	Physcomitrella patens subsp. patens
e_gw1.68.161.1	Physcomitrella patens subsp. patens
e_gw1.81.32.1	Physcomitrella patens subsp. patens
estExt_Genewise1.C_250096	Physcomitrella patens subsp. patens
estExt_gwp_gw1.C_1410051	Physcomitrella patens subsp. patens
29825.m000321	Ricinus communis
30099.m001645	Ricinus communis
30128.m008736	Ricinus communis
30170.m013653	Ricinus communis
e_gw1.3.147.1	Thalassiosira pseudonana CCMP1335
e_gw1.6.287.1	Thalassiosira pseudonana CCMP1335
estExt_gwp_gw1.C_chr_30385	Thalassiosira pseudonana CCMP1335
XP_002952459	Volvox carteri f. nagariensis
XP_002952733	Volvox carteri f. nagariensis
XP_002953598	Volvox carteri f. nagariensis

Cross Link

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PIRSF	PIRSF006468	1.4E-148	32	388	IPR005786	Branched-chain amino acid aminotransferase II
SUPERFAMILY	SSF56752	7.5E-101	50	384	IPR001544	Aminotransferase, class IV
PANTHER	PTHR11825	4.6E-167	53	388	IPR001544	Aminotransferase, class IV
TIGRFAM	TIGR01123	1.3E-97	83	386	IPR005786	Branched-chain amino acid aminotransferase II
Pfam	PF01063	4.2E-27	126	365	IPR001544	Aminotransferase, class IV
ProSitePatterns	PS00770	NA	271	300	IPR018300	Aminotransferase, class IV, conserved site

Subcellular Localization

Localization 1
	Localization	cytosol
	Evidence	SUBAcon
	Pubmed ID	23180787

Localization 2
	Localization	mitochondrion
	Evidence	SUBAcon
	Pubmed ID	23180787

Localization 3
	Localization	plastid
	Evidence	SUBAcon
	Pubmed ID	23180787