Basic information
Locus name	AT4G33150
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling
Effect for Senescence	unclear
Gene Description	lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme identical to lysine-ketoglutarate reductase/saccharopine dehydrogenase GI:2052508 from [Arabidopsis thaliana]
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process oxidation-reduction process molecular function oxidoreductase activity
Sequence	AT4G33150.1 | Genomic | mRNA | CDS | Protein AT4G33150.2 | Genomic | mRNA | CDS | Protein AT4G33150.3 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT4G33150.1  miRNA: ath-miR406 miRNA: ath-miR406 mfe: -23.3 kcal/mol  p-value: 0.026505  position:  1244  target 5' A     G G        A   U 3'             UGGAU G GAUGGCGU CUA              ACCUA U UUAUCGUA GAU   miRNA  3' G     A G        A     5'  target: AT4G33150.1  miRNA: ath-miR779 miRNA: ath-miR779 mfe: -25.4 kcal/mol  p-value: 0.010175  position:  1495  target 5' C C               C  G 3'             G CAACGGGGUUUCCAG CA              C GUUGCUUUAAAGGUU GU   miRNA  3' U A               A    5'  target: AT4G33150.1  miRNA: ath-miR837-5p miRNA: ath-miR837-5p mfe: -22.7 kcal/mol  p-value: 0.135086  position:  2990  target 5' G             C      G 3'             GAAAUGAACAGGA A UGGU              CUUUGCUUGUUCU U ACUA   miRNA  3' A             U G      5'  target: AT4G33150.2  miRNA: ath-miR406 miRNA: ath-miR406 mfe: -23.3 kcal/mol  p-value: 0.026736  position:  1270  target 5' A     G G        A   U 3'             UGGAU G GAUGGCGU CUA              ACCUA U UUAUCGUA GAU   miRNA  3' G     A G        A     5'  target: AT4G33150.2  miRNA: ath-miR779 miRNA: ath-miR779 mfe: -25.4 kcal/mol  p-value: 0.010273  position:  1521  target 5' C C               C  G 3'             G CAACGGGGUUUCCAG CA              C GUUGCUUUAAAGGUU GU   miRNA  3' U A               A    5'  target: AT4G33150.2  miRNA: ath-miR837-5p miRNA: ath-miR837-5p mfe: -22.7 kcal/mol  p-value: 0.135910  position:  3016  target 5' G             C      G 3'             GAAAUGAACAGGA A UGGU              CUUUGCUUGUUCU U ACUA   miRNA  3' A             U G      5'  target: AT4G33150.3  miRNA: ath-miR837-5p miRNA: ath-miR837-5p mfe: -22.7 kcal/mol  p-value: 0.075708  position:  1317  target 5' G             C      G 3'             GAAAUGAACAGGA A UGGU              CUUUGCUUGUUCU U ACUA   miRNA  3' A             U G      5'
Ortholog Group
Ortholog Groups: OG5_129257	Accession Taxon NP_001154283 Arabidopsis thaliana NP_567914 Arabidopsis thaliana NP_849486 ( AT4G33150 ) Arabidopsis thaliana NP_001048324 Oryza sativa Japonica Group estExt_fgenesh1_pm.C_2140008 Physcomitrella patens subsp. patens 29739.m003668 Ricinus communis 29739.m003672 Ricinus communis thaps1_ua_kg.chr_1000384 Thalassiosira pseudonana CCMP1335
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11133 0.0 1 482 No hit NA Pfam PF03435 1.9E-82 7 475 IPR005097 Saccharopine dehydrogenase / Homospermidine synthase SUPERFAMILY SSF51735 3.0E-23 7 201 No hit NA SUPERFAMILY SSF55347 2.2E-75 147 423 No hit NA
Subcellular Localization
Localization 1 Localization other cytoplasmic components Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization cytosol Evidence SUBAcon Pubmed ID 23180787
Localization 3 Localization mitochondrion Evidence SUBAcon Pubmed ID 23180787