Basic information
Locus name	AT1G06520
Alias	GPAT1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Lipid/Carbohydrate metabolism
Effect for Senescence	unclear
Gene Description	phospholipid/glycerol GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 1 (GPAT1); FUNCTIONS IN: 1-acylglycerol-3-phosphate O-acyltransferase activity, acyltransferase activity; INVOLVED IN: pollen sperm cell differentiation, metabolic process. Gene expression is increased during leaf senescence.
Evidence	Genomic evidence:microarray data [Ref 1, Ref 2]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85 2: Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation. Plant Cell 2011 Mar;23(3):873-94
Gene Ontology	biological process metabolic process molecular function transferase activity, transferring acyl groups
Sequence	AT1G06520.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G06520.1  miRNA: ath-miR837-3p miRNA: ath-miR837-3p mfe: -24.8 kcal/mol  p-value: 0.000515  position:  1817  target 5' U         U            G 3'             UCAUUAGUU UUUUGUUUGUUU              GGUAGUCAA AAAACAAGCAAA   miRNA  3'                          5'  target: AT1G06520.1  miRNA: ath-miR867 miRNA: ath-miR867 mfe: -21.9 kcal/mol  p-value: 0.047469  position:  97  target 5' C      C           A   A 3'             UUCUUG UAAAUCAUGUU CAG              AAGGAU AUUUGGUACAA GUU   miRNA  3'        U                 5'
Ortholog Group
Ortholog Groups: OG5_212999	Accession Taxon NP_563768 ( AT1G06520 ) Arabidopsis thaliana 28350.m000105 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR31347 5.0E-217 41 585 No hit NA SUPERFAMILY SSF69593 9.3E-29 310 584 No hit NA Pfam PF01553 3.3E-8 383 488 IPR002123 Phospholipid/glycerol acyltransferase SMART SM00563 1.6E-14 397 498 IPR002123 Phospholipid/glycerol acyltransferase
Subcellular Localization
Localization 1 Localization mitochondria Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization mitochondrion Evidence SUBAcon Pubmed ID 23180787