Basic information
Locus name	AT4G25000
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Lipid/Carbohydrate metabolism
Effect for Senescence	unclear
Gene Description	alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative similar to alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil GI:21670851; contains Pfam profile PF00128: Alpha amylase, catalytic domain
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process carbohydrate metabolic process molecular function alpha-amylase activity calcium ion binding catalytic activity
Pathway	KEGG map00500 (EC 3.2.1.1) MetaCyc PWY-842 Reactome REACT_474
Sequence	AT4G25000.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_132033	Accession Taxon NP_178563 Arabidopsis thaliana NP_567714 ( AT4G25000 ) Arabidopsis thaliana 118171 Chlamydomonas reinhardtii ACO61413 Micromonas sp. RCC299 ACO67331 Micromonas sp. RCC299 NP_001048220 Oryza sativa Japonica Group NP_001058568 Oryza sativa Japonica Group NP_001062023 Oryza sativa Japonica Group NP_001062024 Oryza sativa Japonica Group NP_001063367 Oryza sativa Japonica Group NP_001063368 Oryza sativa Japonica Group NP_001063369 Oryza sativa Japonica Group e_gw1.13.241.1 Physcomitrella patens subsp. patens fgenesh1_pm.scaffold_223000012 Physcomitrella patens subsp. patens 30170.m013877 Ricinus communis 30170.m013878 Ricinus communis XP_002954444 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PIRSF PIRSF001028 0.0 1 423 IPR013775 Alpha-amylase, plant PANTHER PTHR10357 8.5E-147 16 422 IPR015902 Glycoside hydrolase, family 13 SMART SM00642 1.4E-52 26 361 IPR006589 Glycosyl hydrolase, family 13, subfamily, catalytic domain SUPERFAMILY SSF51445 10.0E-71 28 361 IPR017853 Glycoside hydrolase, superfamily Pfam PF00128 8.3E-24 47 342 IPR006047 Glycosyl hydrolase, family 13, catalytic domain PRINTS PR00110 3.6E-5 106 117 IPR006046 Alpha amylase PRINTS PR00110 3.6E-5 197 208 IPR006046 Alpha amylase PRINTS PR00110 3.6E-5 302 314 IPR006046 Alpha amylase Pfam PF07821 1.2E-21 362 422 IPR012850 Alpha-amylase, C-terminal beta-sheet SMART SM00810 6.2E-35 362 422 IPR012850 Alpha-amylase, C-terminal beta-sheet SUPERFAMILY SSF51011 5.8E-18 366 422 No hit NA
Subcellular Localization
Localization	extracellular
Evidence	SUBAcon
Pubmed ID	23180787