Basic information
Locus name	AT3G44880
Alias	PAO/ACD1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Chlorophyll degradation
Effect for Senescence	promote
Gene Description	Encodes a pheide a oxygenase (PAO).
Evidence	Genetic evidence:Mutant [Ref 1, Ref 2]; Genomic evidence:microarray data [Ref 3]
References	1: Tanaka R, Hirashima M, Satoh S, Tanaka A The Arabidopsis-accelerated cell death gene ACD1 is involved in oxygenation of pheophorbide a: inhibition of the pheophorbide a oxygenase activity does not lead to the Plant Cell Physiol. 2003 Dec;44(12):1266-74 2: Pruzinska A, Tanner G, Aubry S, Anders I, Moser S, Muller T, Ongania KH, Kr_utler B, Youn JY, Liljegren SJ, H_rtensteiner S Chlorophyll breakdown in senescent Arabidopsis leaves. Characterization of chlorophyll catabolites and of chlorophyll catabolic enzymes involved in the degreening reaction. Plant Physiol. 2005 Sep;139(1):52-63 3: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process oxidation-reduction process molecular function 2 iron, 2 sulfur cluster binding chlorophyllide a oxygenase [overall] activity oxidoreductase activity
Pathway	KEGG map00860 (EC 1.13.12.14) MetaCyc PWY-5068
Sequence	AT3G44880.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name acd1/pao Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type EMS
Mutated 2 Mutant name 35S-PAO-GFP Mutant/Transgenic transgenic Ecotype Col-0 Mutagenesis type transgene
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_135177	Accession Taxon NP_190074 ( AT3G44880 ) Arabidopsis thaliana 113560 Chlamydomonas reinhardtii 150538 Chlamydomonas reinhardtii 150540 Chlamydomonas reinhardtii 195950 Chlamydomonas reinhardtii ACO64650 Micromonas sp. RCC299 ACO65107 Micromonas sp. RCC299 NP_001048958 Oryza sativa Japonica Group 1000010277 Ostreococcus tauri estExt_fgenesh1_pg.C_Chr_15.00010017 Ostreococcus tauri e_gw1.111.26.1 Physcomitrella patens subsp. patens e_gw1.312.16.1 Physcomitrella patens subsp. patens 29589.m001272 Ricinus communis estExt_thaps1_ua_kg.C_chr_40148 Thalassiosira pseudonana CCMP1335 fgenesh1_pg.C_bd_13x1000019 Thalassiosira pseudonana CCMP1335 XP_002946475 Volvox carteri f. nagariensis XP_002947183 Volvox carteri f. nagariensis XP_002948667 Volvox carteri f. nagariensis XP_002948668 Volvox carteri f. nagariensis XP_002948669 Volvox carteri f. nagariensis XP_002955263 Volvox carteri f. nagariensis XP_002957607 Volvox carteri f. nagariensis XP_002958953 Volvox carteri f. nagariensis XP_002958954 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR21266 5.3E-238 1 476 No hit NA SUPERFAMILY SSF50022 3.0E-33 77 208 IPR017941 Rieske [2Fe-2S] iron-sulphur domain Pfam PF00355 1.2E-18 87 170 IPR017941 Rieske [2Fe-2S] iron-sulphur domain ProSiteProfiles PS51296 30.2 88 200 IPR017941 Rieske [2Fe-2S] iron-sulphur domain SUPERFAMILY SSF55961 3.9E-22 195 433 No hit NA Pfam PF08417 1.3E-26 296 390 IPR013626 Pheophorbide a oxygenase
Subcellular Localization
Localization	plastid
Evidence	SUBAcon
Pubmed ID	23180787