Basic information
Locus name	AT1G26670
Alias	VTI12/SYP41/SYP61/VTI1B
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling
Effect for Senescence	delay
Gene Description	member of VTI1 Gene Family. Normally localizes to the transgolgi network and plasma membrane. A dominant mutation (zip1) alters the subcellular localization of VTI12 and suppresses loss of function mutation (zag1) of VTI11. Interacts with members of the SYP family. Involved in protein trafficking to protein storage vacuoles.under nutrient-poor conditions, vti12 showed an accelerated senescence phenotype
Evidence	Genetic evidence:Mutant [Ref 1, Ref 2, Ref 3, Ref 4, Ref 5, Ref 6]; Genomic evidence:microarray data [Ref 7]
References	1: Zouhar J, Rojo E, Bassham DC AtVPS45 is a positive regulator of the SYP41/SYP61/VTI12 SNARE complex involved in trafficking of vacuolar cargo. Plant Physiol. 2009 Apr;149(4):1668-78 2: Sanmartin M, Ordo_ez A, Sohn EJ, Robert S, Sanchez-Serrano JJ, Surpin MA, Raikhel NV, Rojo E Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 2007 Feb 27;104(9):3645-50 3: Chen Y, Shin YK, Bassham DC YKT6 is a core constituent of membrane fusion machineries at the Arabidopsis trans-Golgi network. J. Mol. Biol. 2005 Jul 1;350(1):92-101 4: Niihama M, Uemura T, Saito C, Nakano A, Sato MH, Tasaka M, Morita MT Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis. Curr. Biol. 2005 Mar 29;15(6):555-60 5: Surpin M, Zheng H, Morita MT, Saito C, Avila E, Blakeslee JJ, Bandyopadhyay A, Kovaleva V, Carter D, Murphy A, Tasaka M, Raikhel N The VTI family of SNARE proteins is necessary for plant viability and mediates different protein transport pathways. Plant Cell 2003 Dec;15(12):2885-99 6: Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell. Mol. Biol. Cell 2001 Dec;12(12):3733-43 7: Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation. Plant Cell 2011 Mar;23(3):873-94
Gene Ontology	biological process intracellular protein transport vesicle-mediated transport cellular component membrane
Sequence	AT1G26670.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	vti12
Mutant/Transgenic	mutant
Ecotype	Col-gl
Mutagenesis type	T-DNA insertion_knock out
Ortholog Group
Ortholog Groups: OG5_127758	Accession Taxon NP_198767 Arabidopsis thaliana NP_564255 ( AT1G26670 ) Arabidopsis thaliana CMJ056C Cyanidioschyzon merolae strain 10D 187998 Chlamydomonas reinhardtii ACO62722 Micromonas sp. RCC299 NP_001043348 Oryza sativa Japonica Group NP_001044021 Oryza sativa Japonica Group 0400010231 Ostreococcus tauri estExt_Genewise1.C_20017 Physcomitrella patens subsp. patens estExt_gwp_gw1.C_4820006 Physcomitrella patens subsp. patens 29635.m000455 Ricinus communis 29813.m001538 Ricinus communis 30138.m004042 Ricinus communis estExt_thaps1_ua_kg.C_chr_40074 Thalassiosira pseudonana CCMP1335 thaps1_ua_kg.chr_23000017 Thalassiosira pseudonana CCMP1335 XP_002949312 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR21230 3.6E-134 3 220 No hit NA SUPERFAMILY SSF47661 7.9E-25 6 93 IPR010989 t-SNARE Pfam PF05008 2.0E-25 12 91 IPR007705 Vesicle transport v-SNARE, N-terminal Coils Coil NA 68 96 No hit NA Pfam PF12352 2.5E-23 129 194 No hit NA SUPERFAMILY SSF58038 7.4E-17 131 190 No hit NA
Subcellular Localization
Localization 1 Localization plasma membrane Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization golgi Evidence SUBAcon Pubmed ID 23180787