Basic information
Locus name	AT1G74710
Alias	SID2/ATICS1/EDS16
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Hormone response pathway:SA
Effect for Senescence	promote
Gene Description	Encodes a protein with isochorismate synthase activity. Mutants fail to accumulate salicylic acid.
Evidence	Genetic evidence:Mutant [Ref 1, Ref 2]; Genomice evidence: microarray data [Ref 3]
References	1: Abreu ME, Munne-Bosch S Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana. J. Exp. Bot. 2009;60(4):1261-71 2: Morris K, MacKerness SA, Page T, John CF, Murphy AM, Carr JP, Buchanan-Wollaston V Salicylic acid has a role in regulating gene expression during leaf senescence. Plant J. 2000 Sep;23(5):677-85 3: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process biosynthetic process molecular function isochorismate synthase activity
Pathway	KEGG map00130 (EC 5.4.4.2) MetaCyc PWY-5837
Sequence	AT1G74710.1 | Genomic | mRNA | CDS | Protein AT1G74710.2 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	sid2
Mutant/Transgenic	mutant
Ecotype	Col-0
Mutagenesis type	T-DNA
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G74710.1  miRNA: ath-miR773 miRNA: ath-miR773 mfe: -30.1 kcal/mol  p-value: 0.003861  position:  1689  target 5' A       U            C 3'             GGGAUAG AGCUGGAAGUGA              CUCUGUU UCGACCUUCGUU   miRNA  3'         U            U 5'  target: AT1G74710.1  miRNA: ath-miR835-5p miRNA: ath-miR835-5p mfe: -26.1 kcal/mol  p-value: 0.003640  position:  1569  target 5' C      G              C 3'             GAUAGA GAAUGUAUGCGGGA              CUAUUU CUUGUAUACGUUCU   miRNA  3'                       U 5'  target: AT1G74710.2  miRNA: ath-miR773 miRNA: ath-miR773 mfe: -30.1 kcal/mol  p-value: 0.004014  position:  1688  target 5' A       U            C 3'             GGGAUAG AGCUGGAAGUGA              CUCUGUU UCGACCUUCGUU   miRNA  3'         U            U 5'  target: AT1G74710.2  miRNA: ath-miR835-5p miRNA: ath-miR835-5p mfe: -26.1 kcal/mol  p-value: 0.003764  position:  1568  target 5' C      G              C 3'             GAUAGA GAAUGUAUGCGGGA              CUAUUU CUUGUAUACGUUCU   miRNA  3'                       U 5'
Ortholog Group
Ortholog Groups: OG5_132914	Accession Taxon NP_001154351 Arabidopsis thaliana NP_173321 Arabidopsis thaliana NP_565090 Arabidopsis thaliana NP_974143 ( AT1G74710 ) Arabidopsis thaliana CMV114C Cyanidioschyzon merolae strain 10D 120595 Chlamydomonas reinhardtii NP_001062984 Oryza sativa Japonica Group e_gw1.24.30.1 Physcomitrella patens subsp. patens 30147.m014077 Ricinus communis thaps1_ua_kg.chr_8000085 Thalassiosira pseudonana CCMP1335 XP_002954494 Volvox carteri f. nagariensis XP_002954495 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11236 1.5E-303 12 560 No hit NA SUPERFAMILY SSF56322 1.2E-80 154 559 IPR005801 ADC synthase TIGRFAM TIGR00543 1.7E-124 199 556 IPR004561 Isochorismate synthase Pfam PF00425 2.4E-60 295 549 IPR015890 Chorismate binding, C-terminal
Mutant Image
mutation of SID2 delays leaf senescence.
Subcellular Localization
Localization	plastid
Evidence	SUBAcon
Pubmed ID	23180787