Basic information
GenBank ID	AB037595
Alias	OsGS1;1
Organism	Rice (Oryza sativa)
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:cytosolic glutamine synthetase
Effect for Senescence	delay
Gene Description	Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1
Evidence	Genetic evidence:Mutant [Ref 1, Ref 2, Ref 3, Ref 4, Ref 5, Ref 6, Ref 7, Ref 8]
References	1: Kamachi K, Yamaya T, Mae T, Ojima K A Role for Glutamine Synthetase in the Remobilization of Leaf Nitrogen during Natural Senescence in Rice Leaves. Plant Physiol. 1991 Jun;96(2):411-7 2: Tabuchi M, Sugiyama K, Ishiyama K, Inoue E, Sato T, Takahashi H, Yamaya T Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1. Plant J. 2005 Jun;42(5):641-51 3: Kamachi K, Yamaya T, Hayakawa T, Mae T, Ojima K Changes in Cytosolic Glutamine Synthetase Polypeptide and its mRNA in a Leaf Blade of Rice Plants during Natural Senescence. Plant Physiol. 1992 Apr;98(4):1323-9 4: Kamachi K, Yamaya T, Hayakawa T, Mae T, Ojima K Vascular bundle-specific localization of cytosolic glutamine synthetase in rice leaves. Plant Physiol. 1992 Aug;99(4):1481-6 5: Sakurai N, Katayama Y, Yamaya T Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice. Physiol Plant 2001 Nov;113(3):400-408 6: Ishiyama K, Inoue E, Tabuchi M, Yamaya T, Takahashi H Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots. Plant Cell Physiol. 2004 Nov;45(11):1640-7 7: Sun H, Huang QM, Su J Highly effective expression of glutamine synthetase genes GS1 and GS2 in transgenic rice plants increases nitrogen-deficiency tolerance. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 2005 Oct;31(5):492-8 8: Cai H, Zhou Y, Xiao J, Li X, Zhang Q, Lian X Overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice. Plant Cell Rep. 2009 Mar;28(3):527-37
Gene Ontology	biological process glutamine biosynthetic process nitrogen compound metabolic process molecular function glutamate-ammonia ligase activity
Pathway	KEGG map00250 (EC 6.3.1.2) map00330 (EC 6.3.1.2) map00910 (EC 6.3.1.2) MetaCyc PWY-3282
Sequence	AB037595.1 | mRNA | CDS | Protein
Mutant information
Mutant name	OsGS1;1-knockout mutant
Mutant/Transgenic	transgenic
Ecotype	rice (O. sativa L. cv. Nipponbare)
Mutagenesis type	En-1/Spm-transposon
miRNA Interaction
Details	target: AB037595  miRNA: osa-miR812a miRNA: osa-miR812a mfe: -27.3 kcal/mol  p-value: 0.008152  position:  607  target 5' U              C       G 3'             UCCAA G UUGGCC GUCUGUC              AGGUU C AAUUGG CAGGCAG   miRNA  3' C     G A                5'  target: AB037595  miRNA: osa-miR812b miRNA: osa-miR812b mfe: -27.3 kcal/mol  p-value: 0.006779  position:  607  target 5' U              C       G 3'             UCCAA G UUGGCC GUCUGUC              AGGUU C AAUUGG CAGGCAG   miRNA  3' C     G A                5'  target: AB037595  miRNA: osa-miR812c miRNA: osa-miR812c mfe: -27.3 kcal/mol  p-value: 0.007373  position:  607  target 5' U              C       G 3'             UCCAA G UUGGCC GUCUGUC              AGGUU C AAUUGG CAGGCAG   miRNA  3' C     G A                5'  target: AB037595  miRNA: osa-miR812d miRNA: osa-miR812d mfe: -27.3 kcal/mol  p-value: 0.008247  position:  607  target 5' U              C       G 3'             UCCAA G UUGGCC GUCUGUC              AGGUU C AAUUGG CAGGCAG   miRNA  3' C     G A                5'  target: AB037595  miRNA: osa-miR812e miRNA: osa-miR812e mfe: -27.3 kcal/mol  p-value: 0.008101  position:  607  target 5' U              C       G 3'             UCCAA G UUGGCC GUCUGUC              AGGUU C AAUUGG CAGGCAG   miRNA  3' C     G A                5'
Ortholog Group
Ortholog Groups: OG5_127569	Accession Taxon NP_001031969 Arabidopsis thaliana NP_001078639 Arabidopsis thaliana NP_175280 Arabidopsis thaliana NP_176794 Arabidopsis thaliana NP_188409 Arabidopsis thaliana NP_198413 Arabidopsis thaliana NP_198576 Arabidopsis thaliana NP_568335 Arabidopsis thaliana CMI233C Cyanidioschyzon merolae strain 10D 129468 Chlamydomonas reinhardtii 133971 Chlamydomonas reinhardtii 136895 Chlamydomonas reinhardtii ACO63243 Micromonas sp. RCC299 NP_001048045 ( AB037595 ) Oryza sativa Japonica Group NP_001049424 Oryza sativa Japonica Group NP_001051067 Oryza sativa Japonica Group NP_001054133 Oryza sativa Japonica Group e_gw1.241.101.1 Physcomitrella patens subsp. patens e_gw1.40.194.1 Physcomitrella patens subsp. patens estExt_fgenesh1_kg.C_190003 Physcomitrella patens subsp. patens estExt_fgenesh1_pg.C_190154 Physcomitrella patens subsp. patens estExt_fgenesh1_pm.C_3450002 Physcomitrella patens subsp. patens estExt_gwp_gw1.C_3450001 Physcomitrella patens subsp. patens 29675.m000392 Ricinus communis 29726.m003921 Ricinus communis estExt_fgenesh1_kg.C_chr_220004 Thalassiosira pseudonana CCMP1335 XP_002947417 Volvox carteri f. nagariensis XP_002947554 Volvox carteri f. nagariensis XP_002956198 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR20852 3.5E-186 9 352 No hit NA SUPERFAMILY SSF54368 1.1E-18 15 102 IPR008147 Glutamine synthetase, beta-Grasp Pfam PF03951 1.8E-19 20 97 IPR008147 Glutamine synthetase, beta-Grasp ProSitePatterns PS00180 NA 55 72 IPR027302 Glutamine synthetase, N-terminal conserved site SUPERFAMILY SSF55931 5.7E-69 103 351 No hit NA Pfam PF00120 2.1E-63 104 350 IPR008146 Glutamine synthetase, catalytic domain ProSitePatterns PS00181 NA 237 253 IPR027303 Glutamine synthetase, glycine-rich site