Basic information
Locus name	AT5G59220
Alias	SAG113
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Hormone response pathway:ABA
Effect for Senescence	promote
Gene Description	Protein phosphatase 2C-like ABA-induced protein. Delayed leaf senescence in SAG113 mutant and premature senescence in overexpressing line.
Evidence	Genomic evidence:microarray data [Ref 1]; Genetic evidence [Ref 2, Ref 3]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85 2: Zhang K, Xia X, Zhang Y, Gan SS An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis. Plant J. 2012 Feb;69(4):667-78 3: Zhang K, Gan SS An abscisic acid-AtNAP transcription factor-SAG113 protein phosphatase 2C regulatory chain for controlling dehydration in senescing Arabidopsis leaves. Plant Physiol. 2012 Feb;158(2):961-9
Gene Ontology	molecular function catalytic activity
Sequence	AT5G59220.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name SAG113KO Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 2 Mutant name SAG113in Mutant/Transgenic transgenic Ecotype Col-0 Mutagenesis type transgene
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT5G59220.1  miRNA: ath-miR826 miRNA: ath-miR826 mfe: -24.8 kcal/mol  p-value: 0.049721  position:  750  target 5' U   A C   A            C 3'             GCG G UUC GAGGCCGGACUG              UGC U AGG UUUUGGCCUGAU   miRNA  3' G   A                    5'
Ortholog Group
Ortholog Groups: OG5_160101	Accession Taxon NP_172223 Arabidopsis thaliana NP_180499 Arabidopsis thaliana NP_187748 Arabidopsis thaliana NP_200730 ( AT5G59220 ) Arabidopsis thaliana NP_001044788 Oryza sativa Japonica Group NP_001049674 Oryza sativa Japonica Group 30143.m001179 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR13832 1.7E-166 1 411 IPR015655 Protein phosphatase 2C SMART SM00332 2.5E-94 101 407 IPR001932 Protein phosphatase 2C (PP2C)-like SUPERFAMILY SSF81606 1.3E-75 108 351 IPR001932 Protein phosphatase 2C (PP2C)-like Pfam PF00481 4.0E-64 112 363 IPR001932 Protein phosphatase 2C (PP2C)-like SMART SM00331 0.0 130 409 IPR001932 Protein phosphatase 2C (PP2C)-like SUPERFAMILY SSF81606 1.3E-75 382 410 IPR001932 Protein phosphatase 2C (PP2C)-like
Subcellular Localization
Localization	nucleus
Evidence	SUBAcon
Pubmed ID	23180787