Basic information
Locus name	AT1G32960
Alias	ATSBT3.3
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:proteolysis
Effect for Senescence	Unclear
Gene Description	Subtilase family protein. Negative regulation of catalytic activity.
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process negative regulation of catalytic activity proteolysis molecular function identical protein binding serine-type endopeptidase activity
Sequence	AT1G32960.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G32960.1  miRNA: ath-miR869 miRNA: ath-miR869 mfe: -26.1 kcal/mol  p-value: 0.017518  position:  512  target 5' U    A               A 3'             GACA UGGGGUUGGACCAAU              UUGU GUCUUAACUUGGUUA   miRNA  3' G    G                 5'
Ortholog Group
Ortholog Groups: OG5_132321	Accession Taxon NP_001078370 Arabidopsis thaliana NP_174573 Arabidopsis thaliana NP_193895 Arabidopsis thaliana NP_564412 Arabidopsis thaliana NP_564413 Arabidopsis thaliana NP_564414 ( AT1G32960 ) Arabidopsis thaliana NP_564868 Arabidopsis thaliana NP_564869 Arabidopsis thaliana NP_567358 Arabidopsis thaliana NP_567359 Arabidopsis thaliana NP_567360 Arabidopsis thaliana NP_567361 Arabidopsis thaliana NP_567362 Arabidopsis thaliana NP_567624 Arabidopsis thaliana NP_567625 Arabidopsis thaliana NP_567632 Arabidopsis thaliana NP_567633 Arabidopsis thaliana NP_568255 Arabidopsis thaliana NP_001044505 Oryza sativa Japonica Group NP_001044507 Oryza sativa Japonica Group NP_001044508 Oryza sativa Japonica Group NP_001044509 Oryza sativa Japonica Group NP_001044510 Oryza sativa Japonica Group NP_001046517 Oryza sativa Japonica Group NP_001046518 Oryza sativa Japonica Group NP_001046520 Oryza sativa Japonica Group NP_001046521 Oryza sativa Japonica Group NP_001046522 Oryza sativa Japonica Group NP_001046523 Oryza sativa Japonica Group NP_001052070 Oryza sativa Japonica Group NP_001052071 Oryza sativa Japonica Group NP_001052072 Oryza sativa Japonica Group NP_001052081 Oryza sativa Japonica Group NP_001052082 Oryza sativa Japonica Group NP_001063751 Oryza sativa Japonica Group 30068.m002604 Ricinus communis 30068.m002606 Ricinus communis 30131.m007119 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR10795 0.0 1 777 IPR015500 Peptidase S8, subtilisin-related SUPERFAMILY SSF52743 7.9E-80 10 157 IPR000209 Peptidase S8/S53 domain Pfam PF05922 5.3E-16 32 108 IPR010259 Proteinase inhibitor I9 PRINTS PR00723 1.0E-13 136 155 IPR015500 Peptidase S8, subtilisin-related Pfam PF00082 2.1E-41 141 623 IPR000209 Peptidase S8/S53 domain SUPERFAMILY SSF52743 7.9E-80 208 353 IPR000209 Peptidase S8/S53 domain PRINTS PR00723 1.0E-13 216 229 IPR015500 Peptidase S8, subtilisin-related ProSitePatterns PS00137 NA 220 230 IPR022398 Peptidase S8, subtilisin, His-active site Pfam PF02225 4.1E-6 401 481 IPR003137 Protease-associated domain, PA SUPERFAMILY SSF52743 7.9E-80 500 688 IPR000209 Peptidase S8/S53 domain PRINTS PR00723 1.0E-13 552 568 IPR015500 Peptidase S8, subtilisin-related ProSitePatterns PS00138 NA 553 563 IPR023828 Peptidase S8, subtilisin, Ser-active site
Subcellular Localization
Localization	extracellular
Evidence	SUBAcon
Pubmed ID	23180787